PMID- 23355198
OWN - NLM
STAT- MEDLINE
DCOM- 20130319
LR  - 20220318
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 139
IP  - 2
DP  - 2013 Feb
TI  - Comparison of fluorescence in situ hybridization (FISH) and dual-ISH (DISH) in 
      the determination of HER2 status in breast cancer.
PG  - 144-50
LID - 10.1309/AJCP13GJAOJAYJMW [doi]
AB  - The determination of HER2 amplification is critical to selecting appropriate 
      patients for HER2 targeted therapy in breast cancer. Dual in situ hybridization 
      (DISH), an alternative to fluorescence in situ hybridization (FISH) and 
      immunohistochemistry, is now available. To compare the FISH and DISH methods, we 
      tested 251 samples enriched for common or difficult-to-assess HER2 anomalies. 
      Seven samples failed DISH testing. There was a 64% (156/244) concordance between 
      FISH and DISH by anomaly (kappa = 0.58, 95% confidence interval, 0.51-0.65; P < 
      .0001) and an 83% (203/244) concordance by amplification status (kappa = 0.58; 95% 
      confidence interval, 0.47-0.69; P < .0001). DISH resulted in lower estimates of 
      HER2/ centromere 17 ratios than FISH, and many cases that were equivocal with 
      FISH were normal with DISH. DISH did not detect any case with coamplification of 
      HER2 and centromere 17. Using a cohort of difficult specimens, we observed less 
      than 95% concordance between FISH and DISH. DISH may underestimate the 
      HER2/chromosome 17 ratio, or FISH may overestimate this ratio.
FAU - Mansfield, Aaron S
AU  - Mansfield AS
AD  - Department of Oncology, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Sukov, William R
AU  - Sukov WR
FAU - Eckel-Passow, Jeanette E
AU  - Eckel-Passow JE
FAU - Sakai, Yuta
AU  - Sakai Y
FAU - Walsh, Frank J
AU  - Walsh FJ
FAU - Lonzo, Melissa
AU  - Lonzo M
FAU - Wiktor, Anne E
AU  - Wiktor AE
FAU - Dogan, Ahmet
AU  - Dogan A
FAU - Jenkins, Robert B
AU  - Jenkins RB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
CIN - Am J Clin Pathol. 2013 Feb;139(2):137-9. PMID: 23355196
MH  - Breast Neoplasms/genetics/*metabolism
MH  - Carcinoma, Ductal, Breast/genetics/*metabolism
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Predictive Value of Tests
MH  - Receptor, ErbB-2/genetics/*metabolism
MH  - Reproducibility of Results
EDAT- 2013/01/29 06:00
MHDA- 2013/03/21 06:00
CRDT- 2013/01/29 06:00
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/03/21 06:00 [medline]
AID - 139/2/144 [pii]
AID - 10.1309/AJCP13GJAOJAYJMW [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2013 Feb;139(2):144-50. doi: 10.1309/AJCP13GJAOJAYJMW.