PMID- 23356671
OWN - NLM
STAT- MEDLINE
DCOM- 20130618
LR  - 20220316
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 13
DP  - 2013 Jan 28
TI  - Electroacupuncture preconditioning reduces cerebral ischemic injury via BDNF and 
      SDF-1alpha in mice.
PG  - 22
LID - 10.1186/1472-6882-13-22 [doi]
AB  - BACKGROUND: This study was designed to determine if electroacupuncture (EA) 
      preconditioning improves tissue outcome and functional outcome following 
      experimentally induced cerebral ischemia in mice. In addition, we investigated 
      whether the expression of brain-derived neurotrophic factor (BDNF) and stromal 
      cell derived factor-1alpha (SDF-1alpha) and infarct volume were related with improvement 
      in neurological and motor function by interventions in this study. METHODS: After 
      treatment with EA at the acupoints 'Baihui (GV20)' and 'Dazhui (GV14)' for 20 
      min, BDNF was assessed in the cortical tissues based on Western blot and the 
      SDF-1alpha and vascular endothelial growth factor (VEGF) levels in the plasma 
      determined by ELISA. To assess the protective effects of EA against ischemic 
      injury, the mice received once a day 20 min EA preconditioning for three days 
      prior to the ischemic event. Focal cerebral ischemia was then induced by 
      photothrombotic cortical ischemia. Infarct volumes, neurobehavioral deficit and 
      motor deficit were evaluated 24 h after focal cerebral ischemia. RESULTS: The 
      expression of BDNF protein increased significantly from 6 h, reaching a plateau 
      at 12 h after the end of EA treatment in the cerebral cortex. Furthermore, 
      SDF-1alpha, not VEGF, increased singnificantly from 12 h to 48 h after EA stimulation 
      in the plasma. Moreover, EA preconditioning reduced the infarct volume by 43.5% 
      when compared to control mice at 24 h after photothrombotic cortical ischemia. 
      Consistent with a smaller infarct size, EA preconditioning showed prominent 
      improvement of neurological function and motor function such as vestibule-motor 
      function, sensori-motor function and asymmetric forelimb use. The expression of 
      BDNF colocalized within neurons and SDF-1alpha colocalized within the cerebral 
      vascular endothelium was observed throughout the ischemic cortex by EA. 
      CONCLUSIONS: Pretreatment with EA increased the production of BDNF and SDF-1alpha, 
      which elicited protective effects against focal cerebral ischemia. These results 
      suggest a novel mechanism of EA pretreatment-induced tolerance against cerebral 
      ischemic injury.
FAU - Kim, Ji Hyun
AU  - Kim JH
AD  - Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan 
      National University, Yangsan, Gyeongnam 626-870, Republic of Korea.
FAU - Choi, Kyung Ha
AU  - Choi KH
FAU - Jang, Young Jung
AU  - Jang YJ
FAU - Kim, Ha Neui
AU  - Kim HN
FAU - Bae, Sun Sik
AU  - Bae SS
FAU - Choi, Byung Tae
AU  - Choi BT
FAU - Shin, Hwa Kyoung
AU  - Shin HK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130128
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - *Acupuncture Points
MH  - Animals
MH  - Brain/*drug effects/metabolism/physiology
MH  - Brain Injuries/metabolism/physiopathology/prevention & control
MH  - Brain Ischemia/metabolism/physiopathology/*therapy
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cerebral Infarction/metabolism/physiopathology/*prevention & control
MH  - Chemokine CXCL12/*metabolism
MH  - *Electroacupuncture
MH  - Endothelium, Vascular/*metabolism
MH  - Forelimb
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Vascular Endothelial Growth Factor A/metabolism
PMC - PMC3562247
EDAT- 2013/01/30 06:00
MHDA- 2013/06/19 06:00
PMCR- 2013/01/28
CRDT- 2013/01/30 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/01/25 00:00 [accepted]
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
PHST- 2013/01/28 00:00 [pmc-release]
AID - 1472-6882-13-22 [pii]
AID - 10.1186/1472-6882-13-22 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2013 Jan 28;13:22. doi: 10.1186/1472-6882-13-22.