PMID- 23357164
OWN - NLM
STAT- MEDLINE
DCOM- 20130918
LR  - 20151119
IS  - 1878-5050 (Electronic)
IS  - 1567-5688 (Linking)
VI  - 14
IP  - 1
DP  - 2013 Jan
TI  - Detection of antibodies in eluates of immunoadsorption causing humoural rejection 
      in patients after solid organ transplantation.
PG  - 191-7
LID - S1567-5688(12)00042-6 [pii]
LID - 10.1016/j.atherosclerosissup.2012.10.031 [doi]
AB  - The influence of antibodies (AB) against human leukocyte antigen (HLA) on 
      antibody mediated rejection (AMR) is still discussed controversially. Here we 
      demonstrate to what extent post transplant detected HLA-AB and non-HLA-AB against 
      Angiotensin II type 1 receptor (AT1 R-AB), endothelin-1 type A receptor (ETA 
      R-AB) and glycoprotein (GP) IIb/IIIa, Ia/IIa, Ib/IX affect the graft outcome. A 
      total of 13 transplant recipients (9 kidneys and 4 hearts) suffering from AMR 
      were analysed. Before immunoadsorption (IA) treatment HLA-AB (CDC) in sera were 
      detected in 27% versus 39% in eluates and 46% versus 87% by using ELISA. We could 
      not find any AB against GP in sera. In eluates, however, we could detect AB 
      against GP: GP IIb/IIIa in 86% of all samples with titres from 1:1 to 1:32, GP 
      Ib/IX (up to 1:32) in 76% and GP Ia/IIa with titres from 1:1 to 1:16 in 82%. 
      Further we detected anti-endothelial cell antibodies (AECA) against receptors AT1 
      and ETA in sera before IA in 22%, after IA in 10% and in eluates in 42% of all 
      samples. The antibody titres vary from 1:1 to 1:256. Our investigation pointed 
      out, that AMR is still possible without detectable AB in serum and consolidates 
      the hypothesis that clinical relevant non-HLA-AB and HLA-AB are partly fixed on 
      the graft. IA is qualified to detach these fixed AB.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Barz, D
AU  - Barz D
AD  - Institute of Transfusion Medicine, University Hospital Jena, Erlanger Allee 3, 
      07747 Jena, Germany. Dagmar.Barz@med.uni-jena.de
FAU - Rummler, S
AU  - Rummler S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Atheroscler Suppl
JT  - Atherosclerosis. Supplements
JID - 100973461
RN  - 0 (AGTR1 protein, human)
RN  - 0 (Antibodies)
RN  - 0 (Biomarkers)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Immunosorbents)
RN  - 0 (Integrin alpha2beta1)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 0 (Receptor, Angiotensin, Type 1)
RN  - 0 (Receptor, Endothelin A)
RN  - 0 (Receptor, Endothelin B)
SB  - IM
MH  - Adsorption
MH  - Antibodies/*blood
MH  - Biomarkers/blood
MH  - Blood Component Removal/*methods
MH  - Graft Rejection/blood/diagnosis/*immunology
MH  - HLA Antigens/immunology
MH  - HLA-B Antigens/immunology
MH  - Heart Transplantation/adverse effects/*immunology
MH  - Humans
MH  - *Immunity, Humoral
MH  - *Immunosorbent Techniques
MH  - Immunosorbents/*therapeutic use
MH  - Integrin alpha2beta1/immunology
MH  - Kidney Transplantation/adverse effects/*immunology
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/immunology
MH  - Platelet Glycoprotein GPIb-IX Complex/immunology
MH  - Receptor, Angiotensin, Type 1/immunology
MH  - Receptor, Endothelin A/immunology
MH  - Receptor, Endothelin B/immunology
MH  - Treatment Outcome
EDAT- 2013/01/30 06:00
MHDA- 2013/09/21 06:00
CRDT- 2013/01/30 06:00
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
AID - S1567-5688(12)00042-6 [pii]
AID - 10.1016/j.atherosclerosissup.2012.10.031 [doi]
PST - ppublish
SO  - Atheroscler Suppl. 2013 Jan;14(1):191-7. doi: 
      10.1016/j.atherosclerosissup.2012.10.031.