PMID- 23357785
OWN - NLM
STAT- MEDLINE
DCOM- 20131024
LR  - 20151119
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 15
IP  - 4
DP  - 2013 Apr
TI  - Protocatechuic acid suppresses ovalbumin-induced airway inflammation in a mouse 
      allergic asthma model.
PG  - 780-8
LID - S1567-5769(13)00026-X [pii]
LID - 10.1016/j.intimp.2013.01.010 [doi]
AB  - Protocatechuic acid (PCA) has been isolated from the leaves of ilex chinenses and 
      has numerous pharmacologic effects, including anti-inflammatory and antitumoral 
      activities. This study aims to evaluate the antiasthma activity of PCA and 
      investigate its possible molecular mechanisms. BALB/c mice were sensitized and 
      challenged to ovalbumin (OVA).Then mice were intraperitoneally (i.p.) injected 
      with PCA 1h before OVA challenge. We found that PCA treatment at 15 or 30 mg/kg 
      significantly decreased OVA-induced airway hyper-responsiveness (AHR) to inhaled 
      methacholine. Type 2 helper T cell (Th2) cytokines in bronchoalveolar lavage 
      (BAL) fluid, such as interleukin-4 (IL-4), interleukin 5 (IL-5) and 
      interleukin-13 (IL-13), and serum OVA-specific immunoglobulin E (IgE) levels, 
      were also reduced by PCA. Moreover treatment with PCA markedly decreased the 
      number of inflammatory cells in BALF and attenuated OVA-induced mRNA expression 
      of CCl11, CCR3, Muc5ac, acidic mammalian chitinase (AMCase), chitinase 3-like 
      protein 4 (Ym2) and E-selectin in lung tissues, lung histopathological studies 
      showed that PCA inhibited inflammatory cell infiltration and mucus hypersecretion 
      compared with the OVA-induced mice group. We then investigated the possible 
      molecular mechanisms which might be implicated in PCA activity. Our results 
      suggested that the protective effect of PCA might be mediated by the inhibition 
      of the extracellular signal-regulated protein kinase (ERK), p38 Mitogen-activated 
      protein kinase (MAPK) phosphorylation and the nuclear factor-kappaB (NF-kappaB) 
      activation.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wei, Miaomiao
AU  - Wei M
AD  - Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College 
      of Veterinary Medicine, Jilin University, Xi'an Road 5333#, Changchun 130062, 
      China.
FAU - Chu, Xiao
AU  - Chu X
FAU - Guan, Mingfeng
AU  - Guan M
FAU - Yang, Xiaofeng
AU  - Yang X
FAU - Xie, Xianxing
AU  - Xie X
FAU - Liu, Fang
AU  - Liu F
FAU - Chen, Chengzhen
AU  - Chen C
FAU - Deng, Xuming
AU  - Deng X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130126
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Hydroxybenzoates)
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 36R5QJ8L4B (protocatechuic acid)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Anti-Asthmatic Agents/administration & dosage/isolation & 
      purification/*therapeutic use
MH  - Asthma/chemically induced/*drug therapy/*immunology
MH  - Blotting, Western
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology/immunology
MH  - Cytokines/blood/immunology
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/administration & dosage/isolation & 
      purification/*therapeutic use
MH  - Female
MH  - Hydroxybenzoates/administration & dosage/isolation & purification/*therapeutic 
      use
MH  - Immunoglobulin E/blood/immunology
MH  - Lung/drug effects/immunology/pathology
MH  - Methacholine Chloride/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/*immunology
MH  - Respiratory Hypersensitivity/chemically induced/drug therapy/immunology
EDAT- 2013/01/30 06:00
MHDA- 2013/10/25 06:00
CRDT- 2013/01/30 06:00
PHST- 2012/09/08 00:00 [received]
PHST- 2013/01/10 00:00 [revised]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/10/25 06:00 [medline]
AID - S1567-5769(13)00026-X [pii]
AID - 10.1016/j.intimp.2013.01.010 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2013 Apr;15(4):780-8. doi: 10.1016/j.intimp.2013.01.010. 
      Epub 2013 Jan 26.