PMID- 23358562
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130129
LR  - 20211021
IS  - 2044-6055 (Electronic)
IS  - 2044-6055 (Linking)
VI  - 3
IP  - 1
DP  - 2013 Jan 28
TI  - EGFR status in oral squamous cell carcinoma: comparing immunohistochemistry, FISH 
      and CISH detection in a case series study.
LID - 10.1136/bmjopen-2012-002077 [doi]
LID - e002077
AB  - OBJECTIVES: To compare the immunohistochemistry (IHC) expression of epidermal 
      growth factor receptor (EGFR) in oral squamous cell carcinomas (OSCC) with the 
      gene amplification evaluated by fluorescence in situ hybridization (FISH) and 
      chromogenic in situ hybridization (CISH) and their association with 
      clinicopathological parameters. Additionally, we tested the sensibility and 
      specificity of CISH in comparison with FISH. DESIGN: Case series study SETTING: 
      Oral surgery and pathology department in a school of dentistry. PARTICIPANTS: 52 
      patients with histopathological diagnosis of OSCC. METHODS: Tumour tissue samples 
      from 52 patients with OSCC were evaluated by IHC, FISH and CISH using tissue 
      microarray technology. Clinicopathological data from all patients were collected. 
      RESULTS: EGFR+ rates were 53.8% (28/52) by IHC, 5.8% (3/52) by CISH and 15.4% 
      (8/52) by FISH. Amplification detected by CISH and FISH with IHC negative 
      occurred in 3.8% (2/52), and one case (1.9%) showed amplification detected by 
      CISH and FISH and protein overexpression concomitantly. There were 9.6% FISH+ 
      cases with IHC and CISH negative rates and 6/8 (75%) FISH+ and also EGFR+ cases; 
      however, an association between protein expression and gene amplification was not 
      found for both techniques. IHC and FISH rates were not associated with 
      clinicopathological features. CISH+ rates were associated with T3-T4 status. 
      Compared with FISH assay, CISH reached a sensitivity of 37.5% and specificity of 
      100%. CONCLUSIONS: There is no association between EGFR expression and gene 
      amplification in OSCC when the IHC is driven to external epitopes of the protein. 
      Although CISH demonstrates specificity, technical problems may influence 
      sensibility when compared with FISH.
FAU - Bernardes, Vanessa Fatima
AU  - Bernardes VF
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
FAU - Gleber-Netto, Frederico Omar
AU  - Gleber-Netto FO
FAU - Sousa, Silvia Ferreira de
AU  - Sousa SF
FAU - Rocha, Rafael Malagoli
AU  - Rocha RM
FAU - Aguiar, Maria Cassia Ferreira de
AU  - Aguiar MC
LA  - eng
PT  - Journal Article
DEP - 20130128
PL  - England
TA  - BMJ Open
JT  - BMJ open
JID - 101552874
PMC - PMC3563135
EDAT- 2013/01/30 06:00
MHDA- 2013/01/30 06:01
PMCR- 2013/01/28
CRDT- 2013/01/30 06:00
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/01/30 06:01 [medline]
PHST- 2013/01/28 00:00 [pmc-release]
AID - bmjopen-2012-002077 [pii]
AID - 10.1136/bmjopen-2012-002077 [doi]
PST - epublish
SO  - BMJ Open. 2013 Jan 28;3(1):e002077. doi: 10.1136/bmjopen-2012-002077.