PMID- 23358924
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR  - 20211021
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 376
IP  - 1-2
DP  - 2013 Apr
TI  - NPY modulates miR-30a-5p and BDNF in opposite direction in an in vitro model of 
      Alzheimer disease: a possible role in neuroprotection?
PG  - 189-95
LID - 10.1007/s11010-013-1567-0 [doi]
AB  - Using in vitro models of Alzheimer's disease (AD), we found that the toxic 
      effects of amyloid beta 25-35 (Abeta(25-35)) on the neurotrophin brain-derived 
      neurotrophic factor (BDNF) were counteracted by pre-incubation with neuropeptide 
      Y (NPY), a neuropeptide expressed within the central nervous system. Nonetheless, 
      the mechanism of action of NPY on BDNF neuronal production in the presence of Abeta 
      is not known. BDNF expression might be directly regulated by microRNA (miRs), 
      small non-coding DNA fragments that regulate the expression of target genes. 
      Thus, there is the possibility that miRs alterations are present in AD-affected 
      neurons and that NPY influences miR expression. To test this hypothesis, we 
      exposed NPY-pretreated primary rat cortical neurons to Abeta(25-35) and measured 
      miR-30a-5p (a member of the miR-30a family involved in BDNF tuning expression) 
      and BDNF mRNA and protein expression after 24 and 48 h. Our results demonstrated 
      that pre-treatment with NPY decreased miR-30a-5p expression and increased BDNF 
      mRNA and protein expression at 24 and 48 h of incubation with Abeta. Therefore, this 
      study demonstrates that NPY modulates BDNF and its regulating microRNA miR-30a-5p 
      in opposite direction with a mechanism that possibly contributes to the 
      neuroprotective effect of NPY in rat cortical neurons exposed to Abeta.
FAU - Croce, Nicoletta
AU  - Croce N
AD  - Department of Clinical and Behavioral Neurology, IRCCS Santa Lucia Foundation, 
      Rome, Italy.
FAU - Gelfo, Francesca
AU  - Gelfo F
FAU - Ciotti, Maria Teresa
AU  - Ciotti MT
FAU - Federici, Giorgio
AU  - Federici G
FAU - Caltagirone, Carlo
AU  - Caltagirone C
FAU - Bernardini, Sergio
AU  - Bernardini S
FAU - Angelucci, Francesco
AU  - Angelucci F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130129
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN30 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (25-35))
SB  - IM
MH  - Alzheimer Disease/*drug therapy/genetics/metabolism
MH  - Amyloid beta-Peptides/*metabolism/pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cerebellar Cortex/cytology/embryology
MH  - Gene Expression Regulation/drug effects
MH  - MicroRNAs/*metabolism
MH  - Molecular Sequence Data
MH  - Neurons/drug effects/metabolism
MH  - Neuropeptide Y/*pharmacology
MH  - Neuroprotective Agents/*pharmacology
MH  - Peptide Fragments/*metabolism/pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2013/01/30 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/01/30 06:00
PHST- 2012/10/03 00:00 [received]
PHST- 2013/01/18 00:00 [accepted]
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - 10.1007/s11010-013-1567-0 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2013 Apr;376(1-2):189-95. doi: 10.1007/s11010-013-1567-0. Epub 
      2013 Jan 29.