PMID- 23359566
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130130
LR  - 20211021
IS  - 1000-9604 (Print)
IS  - 1993-0631 (Electronic)
IS  - 1000-9604 (Linking)
VI  - 24
IP  - 1
DP  - 2012 Mar
TI  - HLA Class I Expressions on Peripheral Blood Mononuclear Cells in Colorectal 
      Cancer Patients.
PG  - 77-82
LID - 10.1007/s11670-012-0077-z [doi]
AB  - OBJECTIVE: To investigate the expression change of human leukocyte antigen (HLA) 
      class I on human peripheral blood mononuclear cells (PBMCs) at both mRNA and 
      protein levels, and to evaluate its roles in the development of colorectal cancer 
      (CRC). METHODS: In the present study, 50 patients with CRC, 35 patients with 
      benign colorectal lesion and 42 healthy volunteers were enrolled. Expression 
      levels of HLA class I mRNA and protein were determined using real-time 
      quantitative reverse transcription PCR (RT-PCR) and flow cytometry analysis, 
      respectively. RESULTS: The expression levels of HLA class I mRNA and proteins 
      were not influenced by age and gender. The relative ratios of HLA class I mRNA 
      were 0.99+/-0.27 in healthy controls, 0.76+/-0.19 in benign patients, and 0.48+/-0.21 
      in CRC patients. Mean fluorescence intensities of HLA class I were 145.58+/-38.14 
      in healthy controls, 102.05+/-35.98 in benign patients and 87.44+/-34.01 in CRC 
      patients. HLA class I on PBMCs was significantly down-regulated at both mRNA and 
      protein levels in patients with stage III and IV CRC. CRC patients with lymph 
      node metastasis also showed a decreased HLA class I expression at protein level. 
      CONCLUSION: HLA class I expressions on PBMCs are associated with staging of CRC 
      and lymph node metastasis. Monitoring the expression of HLA class I on PBMCs may 
      provide useful information for diagnosis and metastasis judgement of CRC.
FAU - Zhang, Zhi-Mian
AU  - Zhang ZM
AD  - Department of Health Examination Center.
FAU - Guan, Xiao
AU  - Guan X
FAU - Li, Ying-Jie
AU  - Li YJ
FAU - Zhu, Ming-Chen
AU  - Zhu MC
FAU - Yang, Xiao-Jing
AU  - Yang XJ
FAU - Zou, Xiong
AU  - Zou X
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Cancer Res
JT  - Chinese journal of cancer research = Chung-kuo yen cheng yen chiu
JID - 9315242
PMC - PMC3555254
OTO - NOTNLM
OT  - Colorectal cancer
OT  - Flow cytometry
OT  - HLA class I
OT  - Peripheral blood mononuclear cells
OT  - RT-PCR
EDAT- 2013/01/30 06:00
MHDA- 2013/01/30 06:01
PMCR- 2012/03/01
CRDT- 2013/01/30 06:00
PHST- 2011/04/26 00:00 [received]
PHST- 2011/09/06 00:00 [accepted]
PHST- 2013/01/30 06:00 [entrez]
PHST- 2013/01/30 06:00 [pubmed]
PHST- 2013/01/30 06:01 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - cjcr-24-01-077 [pii]
AID - 10.1007/s11670-012-0077-z [doi]
PST - ppublish
SO  - Chin J Cancer Res. 2012 Mar;24(1):77-82. doi: 10.1007/s11670-012-0077-z.