PMID- 23363054
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20131121
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 89
IP  - 6
DP  - 2013 Jun
TI  - Modulatory role of quercetin against gamma radiation-mediated biochemical and 
      morphological alterations of red blood cells.
PG  - 471-81
LID - 10.3109/09553002.2013.767989 [doi]
AB  - PURPOSE: The present work was intended to evaluate the radioprotective effect of 
      quercetin against gamma radiation-induced oxidative stress on red blood cells 
      (RBC). MATERIALS AND METHODS: Swiss albino male mice were treated with quercetin 
      (100 mg/kg body wt) for three consecutive days prior to 5 Gy (60)Co-gamma 
      irradiation. RBC was isolated to estimate the level of intracellular reactive 
      oxygen species (ROS), membrane lipid peroxidation (LPO), intracellular reduced 
      glutathione (GSH), mean corpuscular hemoglobin concentration (MCHC), osmotic 
      fragility and morphological alterations by atomic force microscope (AFM). 
      RESULTS: Irradiation increased intracellular ROS and membrane LPO whereas it 
      decreased the intracellular GSH. Quercetin pretreatment ameliorated these 
      alterations. The MCHC value decreased after irradiation whereas quercetin 
      pretreatment restored it. The average osmotic fragility (H50) and the maximum 
      rate of hemolysis (dH/dC)max increased after irradiation. Quercetin pretreatment 
      decreased the H50 and (dH/dC)max. The AFM study showed that irradiation 
      transformed RBC from biconcave to echinocytes, increased their surface roughness 
      and decreased the vertical distance whereas pretreatment of quercetin 
      significantly prevented both the alterations. CONCLUSIONS: Gamma radiation 
      produced ROS and LPO which rendered oxidative stress and ultimately damaged RBC 
      whereas quercetin ameliorated these changes and protected RBC from 
      radiation-mediated damage.
FAU - Das, Dipesh K R
AU  - Das DK
AD  - Department of Physiology, University of Calcutta, Kolkata, India.
FAU - Chakraborty, Anirban
AU  - Chakraborty A
FAU - Sinha, Mahuya
AU  - Sinha M
FAU - Manna, Krishnendu
AU  - Manna K
FAU - Mukherjee, Dipanwita
AU  - Mukherjee D
FAU - Chakraborty, Anindita
AU  - Chakraborty A
FAU - Bhattacharjee, Sekhar
AU  - Bhattacharjee S
FAU - Dey, Sanjit
AU  - Dey S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130304
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Antioxidants)
RN  - 0 (Radiation-Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Antioxidants/administration & dosage
MH  - Cell Survival/drug effects/physiology/radiation effects
MH  - Cells, Cultured
MH  - Erythrocytes/drug effects/*physiology/radiation effects
MH  - Gamma Rays
MH  - Male
MH  - Mice
MH  - Oxidative Stress/drug effects/*physiology/radiation effects
MH  - Quercetin/*administration & dosage
MH  - Radiation Dosage
MH  - Radiation-Protective Agents/administration & dosage
MH  - Reactive Oxygen Species/*metabolism
MH  - *Whole-Body Irradiation
EDAT- 2013/02/01 06:00
MHDA- 2013/08/06 06:00
CRDT- 2013/02/01 06:00
PHST- 2013/02/01 06:00 [entrez]
PHST- 2013/02/01 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
AID - 10.3109/09553002.2013.767989 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2013 Jun;89(6):471-81. doi: 10.3109/09553002.2013.767989. Epub 
      2013 Mar 4.