PMID- 23364441
OWN - NLM
STAT- MEDLINE
DCOM- 20130821
LR  - 20130131
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 27
IP  - 4
DP  - 2013 Feb 20
TI  - Isotype-switched immunoglobulin G antibodies to HIV Gag proteins may provide 
      alternative or additional immune responses to 'protective' human leukocyte 
      antigen-B alleles in HIV controllers.
PG  - 519-28
LID - 10.1097/QAD.0b013e32835cb720 [doi]
AB  - BACKGROUND: Natural control of HIV infection is associated with CD8 T-cell 
      responses to Gag-encoded antigens of the HIV core and carriage of 'protective' 
      human leukocyte antigen (HLA)-B alleles, but some HIV controllers do not possess 
      these attributes. As slower HIV disease progression is associated with high 
      levels of antibodies to HIV Gag proteins, we have examined antibodies to HIV 
      proteins in controllers with and without 'protective' HLA-B alleles. METHODS: 
      Plasma from 32 HIV controllers and 21 noncontrollers was examined for 
      immunoglobulin G1 (IgG1) and IgG2 antibodies to HIV proteins in virus lysates by 
      western blot assay and to recombinant (r) p55 and gp140 by ELISA. Natural killer 
      (NK) cell-activating antibodies and FcgammaRIIa-binding immune complexes were also 
      assessed. RESULTS: Plasma levels of IgG1 antibodies to HIV Gag (p18, p24, rp55) 
      and Pol-encoded (p32, p51, p66) proteins were higher in HIV controllers. In 
      contrast, IgG1 antibodies to Env proteins were less discriminatory, with only 
      antigp120 levels being higher in controllers. High-level IgG2 antibodies to any 
      Gag protein were most common in HIV controllers not carrying a 'protective' HLA-B 
      allele, particularly HLA-B*57 (P = 0.016). HIV controllers without 'protective' 
      HLA-B alleles also had higher plasma levels of IgG1 antip32 (P = 0.04). NK 
      cell-activating antibodies to gp140 Env protein were higher in elite controllers 
      but did not differentiate HIV controllers with or without 'protective' HLA-B 
      alleles. IgG1 was increased in FcgammaRIIa-binding immune complexes from 
      noncontrollers. CONCLUSION: We hypothesize that isotype-switched (IgG2+) 
      antibodies to HIV Gag proteins and possibly IgG1 antip32 may provide alternative 
      or additional immune control mechanisms to HLA-restricted CD8 T-cell responses in 
      HIV controllers.
FAU - French, Martyn A
AU  - French MA
AD  - School of Pathology and Laboratory Medicine, University of Western Australia, 
      Perth, Western Australia, Australia. martyn.french@uwa.edu.au
FAU - Center, Rob J
AU  - Center RJ
FAU - Wilson, Kim M
AU  - Wilson KM
FAU - Fleyfel, Ibrahim
AU  - Fleyfel I
FAU - Fernandez, Sonia
AU  - Fernandez S
FAU - Schorcht, Anna
AU  - Schorcht A
FAU - Stratov, Ivan
AU  - Stratov I
FAU - Kramski, Marit
AU  - Kramski M
FAU - Kent, Stephen J
AU  - Kent SJ
FAU - Kelleher, Anthony D
AU  - Kelleher AD
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Fc gamma receptor IIA)
RN  - 0 (Immunoglobulin G)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, IgG)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Adaptive Immunity/*immunology
MH  - Alleles
MH  - Blotting, Western
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HIV Seropositivity/*immunology/physiopathology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Immunoglobulin G/*immunology
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Male
MH  - RNA, Viral/immunology
MH  - Receptors, IgG/immunology
MH  - Viral Load
MH  - Virus Replication/immunology
MH  - gag Gene Products, Human Immunodeficiency Virus/*immunology
EDAT- 2013/02/01 06:00
MHDA- 2013/08/22 06:00
CRDT- 2013/02/01 06:00
PHST- 2013/02/01 06:00 [entrez]
PHST- 2013/02/01 06:00 [pubmed]
PHST- 2013/08/22 06:00 [medline]
AID - 00002030-201302200-00003 [pii]
AID - 10.1097/QAD.0b013e32835cb720 [doi]
PST - ppublish
SO  - AIDS. 2013 Feb 20;27(4):519-28. doi: 10.1097/QAD.0b013e32835cb720.