PMID- 23365406
OWN - NLM
STAT- MEDLINE
DCOM- 20130729
LR  - 20131121
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 90 Suppl 4
DP  - 2012 Dec
TI  - Ileal mucosa-associated--but not ileal digesta--bacterial profiles in grower pigs 
      are influenced by nutrition and use of antibiotics for weaner pigs.
PG  - 448-50
LID - 10.2527/jas.54004 [doi]
AB  - A longitudinal study was conducted in pigs to evaluate the effect of nutrition 
      and use of antibiotics for weaner pigs on ileal bacterial microbiota. Forty-eight 
      pigs were weaned at 21 +/- 2 d of age and fed a high complexity diet (High) or low 
      complexity diet (Low) with chlortetracycline (A+) or without chlortetracycline 
      (A-) for 6 wk. All pigs received the same grower diet thereafter. Pigs were 
      killed at week 2, 4, and 8 and ileal digesta as well as a 40-cm section of the 
      distal ileum were taken for extraction of digesta and mucosa-associated bacteria, 
      respectively. Total DNA was extracted using a commercially available kit and PCR 
      amplicons were generated using HDA1-GC and HDA2 primers against the V3 region of 
      the 16S rRNA gene. Denaturing gradient gel electrophoresis (DGGE) of PCR 
      amplicons was used for separation of distinct bacteria communities. Similarities 
      of PCR-DGGE profiles were analyzed with Bionumerics software and the degree of 
      similarity represented by a similarity coefficient. The DNA bands were compared 
      using a positional tolerance of 0.5%. In the digesta, HighA-, LowA+, and LowA- 
      were 47.6, 63.6, and 58.8% similar, respectively, at week 2 and 64.0, 69.2, and 
      66.7% similar, respectively, at week 4. Digesta bacteria were 90.9 (HighA-) and 
      85.7% (LowA-) similar to HighA+ at week 8 but due to a smiling edge of the DGGE 
      gel, the profile for LowA+ was deemed inappropriate for similarity analysis. In 
      the mucosa, HighA-, LowA+, and LowA- were 58.8, 80.0 and 44.4% similar, 
      respectively, compared to HighA+ at week 2; 81.5, 75.0, and 88.9% similar, 
      respectively, at week 4; and 81.8, 76.2, and 78.3% similar, respectively, at week 
      8. The bacterial profile was more similar between weeks 2 and 4 in the digesta 
      than in the mucosa but more similar between weeks 4 and 8 in the mucosa than in 
      the digesta. Postweaning nutrition may have a long-term effect on ileal 
      mucosa-associated--but not ileal digesta--microbiota profiles.
FAU - Levesque, C L
AU  - Levesque CL
AD  - Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario 
      N1G 2W1, Canada.
FAU - Yu, H
AU  - Yu H
FAU - Gong, J
AU  - Gong J
FAU - de Lange, C F M
AU  - de Lange CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Anti-Bacterial Agents)
RN  - WCK1KIQ23Q (Chlortetracycline)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Anti-Bacterial Agents/administration & dosage/pharmacology
MH  - Chlortetracycline/administration & dosage/*pharmacology
MH  - Diet/veterinary
MH  - Gastrointestinal Contents/*microbiology
MH  - Ileum/*microbiology
MH  - Intestinal Mucosa/*microbiology
MH  - Swine/*microbiology
MH  - Weaning
EDAT- 2013/02/13 06:00
MHDA- 2013/07/31 06:00
CRDT- 2013/02/01 06:00
PHST- 2013/02/01 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
AID - 90/Supplement_4/448 [pii]
AID - 10.2527/jas.54004 [doi]
PST - ppublish
SO  - J Anim Sci. 2012 Dec;90 Suppl 4:448-50. doi: 10.2527/jas.54004.