PMID- 23370691
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20211021
IS  - 1473-0189 (Electronic)
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 13
IP  - 7
DP  - 2013 Apr 7
TI  - Development of a single-cell array for large-scale DNA fluorescence in situ 
      hybridization.
PG  - 1316-24
LID - 10.1039/c2lc40364a [doi]
AB  - DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, 
      but conventional sample-preparation methods for FISH do not support large-scale 
      high-throughput data acquisition and analysis, which are potentially useful for 
      several biomedical applications. To address this limitation, we have developed a 
      novel FISH sample-preparation method based on generating a centimetre-sized cell 
      array, in which all cells are precisely positioned and separated from their 
      neighbours. This method is simple and capable of patterning nonadherent human 
      cells. We have successfully performed DNA FISH on the single-cell arrays, which 
      facilitates analysis of the FISH results with the FISH-FINDER computer program.
FAU - Liu, Yingru
AU  - Liu Y
AD  - Department of Chemical and Biomedical Engineering, FAMU-FSU College of 
      Engineering, Florida State University, Tallahassee, Florida 32310-2870, USA.
FAU - Kirkland, Brett
AU  - Kirkland B
FAU - Shirley, James
AU  - Shirley J
FAU - Wang, Zhibin
AU  - Wang Z
FAU - Zhang, Peipei
AU  - Zhang P
FAU - Stembridge, Jacquelyn
AU  - Stembridge J
FAU - Wong, Wilson
AU  - Wong W
FAU - Takebayashi, Shin-ichiro
AU  - Takebayashi S
FAU - Gilbert, David M
AU  - Gilbert DM
FAU - Lenhert, Steven
AU  - Lenhert S
FAU - Guan, Jingjiao
AU  - Guan J
LA  - eng
GR  - P01 GM085354/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - K562 Cells
MH  - Single-Cell Analysis/*methods
MH  - Surface Properties
MH  - Tissue Array Analysis/*methods
PMC - PMC3594524
MID - NIHMS431596
EDAT- 2013/02/02 06:00
MHDA- 2013/08/30 06:00
PMCR- 2013/10/07
CRDT- 2013/02/02 06:00
PHST- 2013/02/02 06:00 [entrez]
PHST- 2013/02/02 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
PHST- 2013/10/07 00:00 [pmc-release]
AID - 10.1039/c2lc40364a [doi]
PST - ppublish
SO  - Lab Chip. 2013 Apr 7;13(7):1316-24. doi: 10.1039/c2lc40364a.