PMID- 23373536
OWN - NLM
STAT- MEDLINE
DCOM- 20131118
LR  - 20130213
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 6
DP  - 2013 Feb 13
TI  - Community of environmental streptomyces related to geosmin development in Chinese 
      liquors.
PG  - 1343-8
LID - 10.1021/jf3040513 [doi]
AB  - Diverse Streptomyces species act as geosmin producers in the Chinese 
      liquor-making process. In this paper, the ecology of these Streptomyces species 
      was analyzed using denaturing gradient gel electrophoresis (DGGE) of amplified 
      Actinobacteria -specified rDNA. The result showed that Streptomyces were widely 
      distributed during Daqu incubation, and multiple processing, geographic, and 
      climate factors can affect their distribution and diversity. The genes associated 
      with geosmin production were characterized in four geosmin-producing Streptomyces 
      strains, all of which were isolated from geosmin-contaminated Daqu. On the basis 
      of this information, a real-time PCR method was developed, enabling the detection 
      of traces of Streptomyces in complex solid-state matrices. The primer was 
      targeted at the gene coding for geosmin synthase (geoA). The real-time PCR method 
      was found to be specific for geosmin-producing Streptomyces and did not show any 
      cross-reactivity with geosmin-negative isolates, which are frequently present in 
      the Chinese liquor-brewing process. Quantification of geoA in the Chinese 
      liquor-making process could permit the monitoring of the level of geosmin 
      producers prior to the occurrence of geosmin production. Comparison of the qPCR 
      results based on the gene encoding geosmin synthase and Actinobacteria-specified 
      rDNA showed that about 1-10% of the Actinobacteria carry the geosmin synthesis 
      gene.
FAU - Du, Hai
AU  - Du H
AD  - State Key Laboratory of Food Science and Technology, Key Laboratory of Industrial 
      Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan 
      University, 1800 Lihu Avenue, Wuxi, Jiangsu, China 214122.
FAU - Lu, Hu
AU  - Lu H
FAU - Xu, Yan
AU  - Xu Y
FAU - Du, Xiaowei
AU  - Du X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130201
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Bacterial Proteins)
RN  - 0 (Naphthols)
RN  - MYW912WXJ4 (geosmin)
SB  - IM
MH  - Actinobacteria/classification/genetics/isolation & purification
MH  - Alcoholic Beverages/*microbiology
MH  - Bacterial Proteins/genetics/metabolism
MH  - *Environmental Microbiology
MH  - Naphthols/*metabolism
MH  - Streptomyces/classification/genetics/*isolation & purification/*metabolism
EDAT- 2013/02/05 06:00
MHDA- 2013/11/19 06:00
CRDT- 2013/02/05 06:00
PHST- 2013/02/05 06:00 [entrez]
PHST- 2013/02/05 06:00 [pubmed]
PHST- 2013/11/19 06:00 [medline]
AID - 10.1021/jf3040513 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Feb 13;61(6):1343-8. doi: 10.1021/jf3040513. Epub 2013 
      Feb 1.