PMID- 23374518
OWN - NLM
STAT- MEDLINE
DCOM- 20130722
LR  - 20151119
IS  - 1681-7168 (Electronic)
IS  - 1022-386X (Linking)
VI  - 23
IP  - 2
DP  - 2013 Feb
TI  - Serum levo-carnitine levels and skeletal muscle functions in type 2 diabetes 
      mellitus in rodents.
PG  - 132-6
AB  - OBJECTIVE: To study serum levo-carnitine (l-carnitine) levels and isometric 
      contraction, force frequency relationship and fatigue of rodent skeletal muscles 
      in type 2 diabetes mellitus. STUDY DESIGN: Randomized controlled trial. PLACE AND 
      DURATION OF STUDY: Physiology Department, Army Medical College, Rawalpindi, from 
      January 2009 to January 2010. METHODOLOGY: Sixty Sprague-Dawley rats were 
      randomly divided into two groups; group I (control), fed on normal diet ad 
      libitum and Group II (diabetic), fed on high fat diet and administered 
      streptozocin to induce type 2 diabetes mellitus (T2DM). At 21st day, plasma 
      glucose and TG/HDL ratio were measured to confirm the development of T2DM in 
      group II. At 28th day, blood was drawn by intracardiac puncture to estimate serum 
      levo-carnitine levels. Contractile functions of skeletal muscles were assessed by 
      using iWorx AHK/214 physiological data acquisition unit. Simple muscle twitches, 
      maximum isometric twitch tension (MITT), time-to-peak twitch tension (TPTT) and 
      time-to-relax to 50% of the peak twitch tension (1/2RT) of extensor digitorum 
      muscles were recorded. Muscles were stimulated at higher frequencies to determine 
      maximum fused tetanic tension (MFTT), maximum fused tetanic tension after fatigue 
      protocol (TTFP) and recovery from fatigue (RF). RESULTS: Serum levo-carnitine 
      level decreased significantly in the diabetic group. Both groups had similar 
      MITT, TPTT and 1/2RT but decline in MFTT, TTFP and RF was significant in the 
      diabetic rats. CONCLUSION: T2DM adversely affected serum levo-carnitine levels 
      and the contractile functions of rodent skeletal muscle at high frequency 
      stimulation.
FAU - Bin Aleem, Shoaib
AU  - Bin Aleem S
AD  - Department of Physiology, Army Medical College, Rawalpindi. shoaib.phy@gmail.com
FAU - Hussain, Muhammad Mazhar
AU  - Hussain MM
FAU - Farooq, Yasir
AU  - Farooq Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Pakistan
TA  - J Coll Physicians Surg Pak
JT  - Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
JID - 9606447
RN  - 5W494URQ81 (Streptozocin)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Animals
MH  - Carnitine/*blood
MH  - Diabetes Mellitus, Experimental/blood/*metabolism/*physiopathology
MH  - Diabetes Mellitus, Type 2/blood/*complications/metabolism
MH  - Diet, High-Fat/adverse effects
MH  - Electric Stimulation
MH  - Isometric Contraction/*drug effects
MH  - Muscle Fatigue/drug effects
MH  - Muscle Fibers, Fast-Twitch
MH  - Muscle Fibers, Slow-Twitch
MH  - Muscle, Skeletal/*drug effects/physiopathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Streptozocin/administration & dosage
EDAT- 2013/02/05 06:00
MHDA- 2013/07/23 06:00
CRDT- 2013/02/05 06:00
PHST- 2010/10/15 00:00 [received]
PHST- 2012/12/03 00:00 [accepted]
PHST- 2013/02/05 06:00 [entrez]
PHST- 2013/02/05 06:00 [pubmed]
PHST- 2013/07/23 06:00 [medline]
AID - 040579197 [pii]
PST - ppublish
SO  - J Coll Physicians Surg Pak. 2013 Feb;23(2):132-6.