PMID- 23374597
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20191112
IS  - 1488-2353 (Electronic)
IS  - 0147-958X (Linking)
VI  - 36
IP  - 1
DP  - 2013 Feb 1
TI  - Association of monocyte CCR2 expression with obesity and insulin resistance in 
      postmenopausal women.
PG  - E24-31
AB  - PURPOSE: Monocytes actively participate in inflammatory mechanisms that 
      contribute to the development of adipose tissue dysfunction and atherogenesis. 
      The aim of this study was to evaluate the association of monocyte CCR2 chemokine 
      receptor expression and intracellular oxidative burst with the metabolic and 
      inflammatory factors related to body weight. METHODS: The study was performed in 
      67 postmenopausal women with normal, overweight and obese body mass index. 
      Monocyte CCR2 surface expression and intracellular oxidative burst activity 
      (determined using 2', 7'-dichlorofluorescin diacetate) were analyzed by flow 
      cytometry. Serum levels of HMW adoponectin, monocyte chemoattractant protein-1 
      (MCP-1), insulin, glucose, lipids and C-reactive protein were determined. 
      RESULTS: Subjects with homeostasis model assessment-estimated insulin resistance 
      (HOMA-IR) above the median had significantly higher proportion of CCR2+ monocytes 
      and higher mean fluorescence intensity (MFI) of CCR2 and oxidative burst. The 
      proportion of CCR2+ monocytes and CCR2 MFI were correlated with body weight, body 
      mass index, fat mass, insulin and HOMA-IR. Oxidative burst also correlated with 
      anthropometric measures, fat mass and expression of CCR2. No correlations were 
      found between these markers of monocyte activation and HMW adiponectin or 
      monocyte chemoattractant protein-1. The absolute number of monocytes was 
      associated with insulin and this association remained significant after adjusting 
      for C-reactive protein. In the multiple regression model the monocyte number was 
      determined to be an independent predictor of insulin level. CONCLUSION: These 
      results provide support for significant associations of monocyte number and 
      markers involved in monocyte activation with obesity and insulin resistance.
FAU - Pandzic Jaksic, Vlatka
AU  - Pandzic Jaksic V
AD  - Department of Endocrinology and Metabolism, Dubrava University Hospital, Zagreb, 
      Croatia. vpandzic@kbd.hr
FAU - Gizdic, Branimir
AU  - Gizdic B
FAU - Miletic, Zorana
AU  - Miletic Z
FAU - Trutin-Ostovic, Karmen
AU  - Trutin-Ostovic K
FAU - Jaksic, Ozren
AU  - Jaksic O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130201
PL  - Canada
TA  - Clin Invest Med
JT  - Clinical and investigative medicine. Medecine clinique et experimentale
JID - 7804071
RN  - 0 (CCR2 protein, human)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - Aged
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Insulin Resistance/*physiology
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Obesity/*metabolism
MH  - Postmenopause/*metabolism
MH  - Receptors, CCR2/*metabolism
EDAT- 2013/02/05 06:00
MHDA- 2013/08/10 06:00
CRDT- 2013/02/05 06:00
PHST- 2013/02/04 00:00 [received]
PHST- 2013/02/05 06:00 [entrez]
PHST- 2013/02/05 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.25011/cim.v36i1.19402 [doi]
PST - epublish
SO  - Clin Invest Med. 2013 Feb 1;36(1):E24-31. doi: 10.25011/cim.v36i1.19402.