PMID- 23376609
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20130318
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 369
IP  - 1-2
DP  - 2013 Apr 30
TI  - Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells.
PG  - 72-85
LID - S0303-7207(13)00031-2 [pii]
LID - 10.1016/j.mce.2013.01.008 [doi]
AB  - Previous results have suggested the existence of receptors for monocyte 
      chemoattractant protein-1 (MCP-1), CC chemokine receptors 2 (CCR2), in human 
      adipocytes and their involvement in mediating effects of MCP-1 on adipocyte 
      functions. However, the presence of CCR2 present on non-adipose-lineage cells of 
      adipose tissue has not been excluded. We have used human 
      Simpson-Golabi-Behmel-Syndrome (SGBS) preadipocytes and in-vitro-differentiated 
      mature adipocytes to investigate the expression of CCR2 in human (pre)adipocytes. 
      We found that the cells are devoid of CCR2 receptor protein and mRNA expression 
      and fail to respond to treatment with all known CCR2 chemokine agonists. CCR2 is 
      also absent from (pre)adipocytes prepared in vitro from human multipotent 
      adipose-derived stem cells, bone-marrow-derived mesenchymal stem cells, or from 
      primary (pre)adipocytes. Conditions mimicking proinflammatory changes in adipose 
      tissue did not induce CCR2 receptor expression. We conclude that CCR2 is absent 
      from human adipose lineage cells. Functional effects previously described for 
      MCP-1 in human adipose tissue may be mediated indirectly through paracrine 
      effects on non-adipose-lineage cells or by a (pre)adipocyte receptor for MCP-1 
      distinct from CCR2.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Koenig, Carolin
AU  - Koenig C
AD  - Institute of Pharmacology and Toxicology, Ulm University Medical Center, Ulm, 
      Germany.
FAU - Fischer-Posovszky, Pamela
AU  - Fischer-Posovszky P
FAU - Rojewski, Markus T
AU  - Rojewski MT
FAU - Tews, Daniel
AU  - Tews D
FAU - Schrezenmeier, Hubert
AU  - Schrezenmeier H
FAU - Wabitsch, Martin
AU  - Wabitsch M
FAU - Gierschik, Peter
AU  - Gierschik P
FAU - Moepps, Barbara
AU  - Moepps B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130129
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - Adipocytes/drug effects/metabolism
MH  - Adipose Tissue/cytology/metabolism
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Humans
MH  - Monocyte Chemoattractant Proteins/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, CCR2/genetics/*metabolism
EDAT- 2013/02/05 06:00
MHDA- 2013/09/17 06:00
CRDT- 2013/02/05 06:00
PHST- 2012/09/10 00:00 [received]
PHST- 2013/01/11 00:00 [revised]
PHST- 2013/01/12 00:00 [accepted]
PHST- 2013/02/05 06:00 [entrez]
PHST- 2013/02/05 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
AID - S0303-7207(13)00031-2 [pii]
AID - 10.1016/j.mce.2013.01.008 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2013 Apr 30;369(1-2):72-85. doi: 10.1016/j.mce.2013.01.008. 
      Epub 2013 Jan 29.