PMID- 23377490 OWN - NLM STAT- MEDLINE DCOM- 20131031 LR - 20221207 IS - 1432-0991 (Electronic) IS - 0343-8651 (Linking) VI - 66 IP - 6 DP - 2013 Jun TI - Analysis of microbial population dynamics in a partial nitrifying SBR at ambient temperature. PG - 614-20 LID - 10.1007/s00284-013-0317-6 [doi] AB - In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 degrees C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature. FAU - Zeng, Taotao AU - Zeng T AD - School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China. biowater@126.com FAU - Li, Dong AU - Li D FAU - Zeng, Huiping AU - Zeng H FAU - Zhang, Zhao AU - Zhang Z FAU - Liu, Liqian AU - Liu L FAU - Zhang, Xiaojing AU - Zhang X FAU - Zhang, Jie AU - Zhang J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130203 PL - United States TA - Curr Microbiol JT - Current microbiology JID - 7808448 RN - 0 (DNA, Bacterial) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Waste Water) RN - 7664-41-7 (Ammonia) SB - IM MH - Ammonia/*metabolism MH - Base Sequence MH - *Bioreactors MH - DNA, Bacterial/analysis/genetics MH - In Situ Hybridization, Fluorescence MH - Microbial Consortia MH - Nitrification MH - Nitrosomonas/genetics/*growth & development/isolation & purification/metabolism MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Temperature MH - *Waste Disposal, Fluid MH - Wastewater/*microbiology MH - Water Purification EDAT- 2013/02/05 06:00 MHDA- 2013/11/01 06:00 CRDT- 2013/02/05 06:00 PHST- 2012/10/28 00:00 [received] PHST- 2013/01/09 00:00 [accepted] PHST- 2013/02/05 06:00 [entrez] PHST- 2013/02/05 06:00 [pubmed] PHST- 2013/11/01 06:00 [medline] AID - 10.1007/s00284-013-0317-6 [doi] PST - ppublish SO - Curr Microbiol. 2013 Jun;66(6):614-20. doi: 10.1007/s00284-013-0317-6. Epub 2013 Feb 3.