PMID- 23378244
OWN - NLM
STAT- MEDLINE
DCOM- 20131114
LR  - 20220408
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Print)
IS  - 1234-1983 (Linking)
VI  - 54
IP  - 2
DP  - 2013 May
TI  - Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (X 
      Triticosecale Witt.) breeding.
PG  - 147-55
LID - 10.1007/s13353-013-0133-5 [doi]
AB  - Chromosome identification using fluorescence in situ hybridization (FISH) is 
      widely used in cytogenetic research. It is a diagnostic tool helpful in 
      chromosome identification. It can also be used to characterize alien 
      introgressions, when exercised in a combination with genomic in situ 
      hybridization (GISH). This work aims to find chromosome identification of 
      Aegilops species and Aegilops x Secale amphiploids, which can be used in cereal 
      breeding as a source of favourable agronomic traits. Four diploid and two 
      tetraploid Aegilops species and three Aegilops x Secale hybrids were analysed 
      using FISH with pSc119.2, pAs1, 5S rDNA and 25S rDNA clones to differentiate the 
      U-, M-, S(sh)- and D-subgenome chromosomes of Aegilops genus. Additionally, GISH 
      for chromosome categorization was carried out. Differences in the hybridization 
      patterns allowed to identify all U-, M-, S(sh)- and D-subgenome chromosomes. Some 
      differences in localization of the rDNA, pSc119.2 and pAs1 sequences between 
      analogue subgenomes in diploid and tetraploid species and Aegilops x Secale 
      hybrids were detected. The hybridization pattern of the M and S genome was more 
      variable than that of the U and D genome. An importance of the cytogenetic 
      markers in plant breeding and their possible role in chromosome structure, 
      function and evolution is discussed.
FAU - Kwiatek, M
AU  - Kwiatek M
AD  - Laboratory of Distant Crosses, Institute of Plant Genetics, Polish Academy of 
      Sciences, Strzeszynska 34, 60-479, Poznan, Poland. mkwi@igr.poznan.pl
FAU - Wisniewska, H
AU  - Wisniewska H
FAU - Apolinarska, B
AU  - Apolinarska B
LA  - eng
PT  - Journal Article
DEP - 20130202
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Plant/chemistry/metabolism
MH  - Edible Grain/*genetics
MH  - Genetic Markers
MH  - Genome, Plant
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Poaceae/genetics
PMC - PMC3620446
EDAT- 2013/02/05 06:00
MHDA- 2013/11/15 06:00
PMCR- 2013/02/02
CRDT- 2013/02/05 06:00
PHST- 2012/10/02 00:00 [received]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/01/09 00:00 [revised]
PHST- 2013/02/05 06:00 [entrez]
PHST- 2013/02/05 06:00 [pubmed]
PHST- 2013/11/15 06:00 [medline]
PHST- 2013/02/02 00:00 [pmc-release]
AID - 133 [pii]
AID - 10.1007/s13353-013-0133-5 [doi]
PST - ppublish
SO  - J Appl Genet. 2013 May;54(2):147-55. doi: 10.1007/s13353-013-0133-5. Epub 2013 
      Feb 2.