PMID- 23379581
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20211021
IS  - 2152-4998 (Electronic)
IS  - 2152-4971 (Print)
IS  - 2152-4971 (Linking)
VI  - 15
IP  - 1
DP  - 2013 Feb
TI  - Adenovector-mediated gene delivery to human umbilical cord mesenchymal stromal 
      cells induces inner ear cell phenotype.
PG  - 43-54
LID - 10.1089/cell.2011.0097 [doi]
AB  - Hearing is one of our main sensory systems and having a hearing disorder can have 
      a significant impact in an individual's quality of life. Sensory neural hearing 
      loss (SNHL) is the most common form of hearing loss; it results from the 
      degeneration of inner ear sensory hair cells and auditory neurons in the cochlea, 
      cells that are terminally differentiated. Stem cell-and gene delivery-based 
      strategies provide an opportunity for the replacement of these cells. In recent 
      years, there has been an increasing interest in gene delivery to mesenchymal stem 
      cells. In this study, we evaluated the potential of human umbilical cord 
      mesenchymal stromal cells (hUCMSCs) as a possible source for regenerating inner 
      ear hair cells. The expression of Atoh1 induced the differentiation of hUCMSCs 
      into cells that resembled inner ear hair cells morphologically and 
      immunocytochemically, evidenced by the expression of hair cell-specific markers. 
      The results demonstrated for the first time that hUCMSCs can differentiate into 
      hair cell-like cells, thus introducing a new potential tissue engineering and 
      cell transplantation approach for the treatment of hearing loss.
FAU - Devarajan, Keerthana
AU  - Devarajan K
AD  - Bioengineering Program, University of Kansas, Lawrence, 66045, USA.
FAU - Forrest, M Laird
AU  - Forrest ML
FAU - Detamore, Michael S
AU  - Detamore MS
FAU - Staecker, Hinrich
AU  - Staecker H
LA  - eng
GR  - R01 CA173292/CA/NCI NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Cell Reprogram
JT  - Cellular reprogramming
JID - 101528176
RN  - 0 (ATOH1 protein, human)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
SB  - IM
MH  - *Adenoviridae
MH  - Basic Helix-Loop-Helix Transcription Factors/biosynthesis/genetics
MH  - *Cell Differentiation
MH  - Cell- and Tissue-Based Therapy
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression/genetics
MH  - Genetic Therapy
MH  - Hair Cells, Auditory, Inner/*cytology/*metabolism
MH  - Hearing Loss/genetics/metabolism/therapy
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cells/cytology/metabolism
MH  - *Transduction, Genetic
MH  - *Umbilical Cord/cytology/metabolism
PMC - PMC4298749
EDAT- 2013/02/06 06:00
MHDA- 2013/07/16 06:00
PMCR- 2014/02/01
CRDT- 2013/02/06 06:00
PHST- 2013/02/06 06:00 [entrez]
PHST- 2013/02/06 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - 10.1089/cell.2011.0097 [pii]
AID - 10.1089/cell.2011.0097 [doi]
PST - ppublish
SO  - Cell Reprogram. 2013 Feb;15(1):43-54. doi: 10.1089/cell.2011.0097.