PMID- 23381392 OWN - NLM STAT- MEDLINE DCOM- 20130821 LR - 20130531 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 158 IP - 6 DP - 2013 Jun TI - A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia. PG - 1235-44 LID - 10.1007/s00705-012-1600-8 [doi] AB - A suspected virus disease was identified from an arborescent Brugmansia x candida Pers. (syn. Datura candida Pers.) tree. The causal agent was aphid transmissible at low rates. Viral particles were purified from infected tobacco tissue, analyzed, and purified virions were inoculated into healthy tobacco plants to recreate the symptoms. The virions had a mean length of 720-729 nm, and infected cells contained inclusion bodies typical of potyvirus infections. Analysis of infected tissues and purified virions with a panel of potyvirus-specific antibodies confirmed identification as a potyvirus. Viral host range, dilution end point, thermal tolerance and aphid transmission characteristics were examined. The viral genome (9761 nt) is typical of potyviruses, with the closest related potyvirus being pepper mottle virus, at 72 % nt sequence identity. Based on conventions for naming novel potyviruses, the virus was determined to be a member of a previously undescribed species, tentatively named "Brugmansia mosaic virus" (BruMV). FAU - Damsteegt, Vernon D AU - Damsteegt VD AD - USDA-ARS Foreign Disease/Weed Science Research Unit, Fort Detrick, MD, USA. FAU - Stone, Andrew L AU - Stone AL FAU - Smith, Oney P AU - Smith OP FAU - McDaniel, Lawrence AU - McDaniel L FAU - Sherman, Diana J AU - Sherman DJ FAU - Dardick, Chris AU - Dardick C FAU - Hammond, John AU - Hammond J FAU - Jordan, Ramon AU - Jordan R FAU - Schneider, William L AU - Schneider WL LA - eng PT - Journal Article DEP - 20130205 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (Antibodies, Viral) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Antibodies, Viral/immunology MH - Aphids/virology MH - Genome, Viral/genetics MH - Microscopy, Electron MH - Phylogeny MH - Plant Diseases/etiology/virology MH - Polymerase Chain Reaction MH - Potyvirus/genetics/immunology/isolation & purification/*physiology/ultrastructure MH - RNA, Viral/genetics MH - Solanaceae/*virology MH - Virion/isolation & purification/physiology EDAT- 2013/02/06 06:00 MHDA- 2013/08/22 06:00 CRDT- 2013/02/06 06:00 PHST- 2012/09/27 00:00 [received] PHST- 2012/12/01 00:00 [accepted] PHST- 2013/02/06 06:00 [entrez] PHST- 2013/02/06 06:00 [pubmed] PHST- 2013/08/22 06:00 [medline] AID - 10.1007/s00705-012-1600-8 [doi] PST - ppublish SO - Arch Virol. 2013 Jun;158(6):1235-44. doi: 10.1007/s00705-012-1600-8. Epub 2013 Feb 5.