PMID- 2338166
OWN - NLM
STAT- MEDLINE
DCOM- 19900621
LR  - 20190828
IS  - 0020-711X (Print)
IS  - 0020-711X (Linking)
VI  - 22
IP  - 4
DP  - 1990
TI  - Mitochondrial hexokinase from differentiated and undifferentiated HT29 colon 
      cancer cells: effect of some metabolites on the bound/soluble equilibrium.
PG  - 419-23
AB  - 1. Solubilization of mitochondrial bound hexokinase (HK), which represents 75-80% 
      of the total enzyme activity in the cells, was investigated in freshly isolated 
      mitochondria from undifferentiated (Glc+) or differentiated (Glc-) HT29 
      adenocarcinoma cells. In both models, the bound HK is almost completely released 
      in vitro by 100 microM glucose 6-P (G 6-P). 2. Free ATP (5 mM) or palmitate (800 
      microM) produce a partial solubilization of bound HK, more markedly in the case 
      of Glc- mitochondria. 3. Glucose or glucose 1-P are found unable to solubilize 
      bound HK. Glucose 1,6-P2, 2-deoxyglucose 6-P or glucosamine 6-P can solubilize 
      the enzyme but are less efficient than G 6-P. 4. Mg2+ and Pi are found to 
      counteract the glucose 6-P induced solubilization of HK in both types of 
      mitochondria. Taking into account the intracellular concentrations of these ions, 
      this could in part explain why, in HT29 cells, HK is predominantly bound to the 
      mitochondria.
FAU - Gauthier, T
AU  - Gauthier T
AD  - Institut de Physiologie, INSERM U 317, Universite Paul Sabatier, Toulouse, 
      France.
FAU - Denis-Pouxviel, C
AU  - Denis-Pouxviel C
FAU - Murat, J C
AU  - Murat JC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Biochem
JT  - The International journal of biochemistry
JID - 0250365
RN  - 0 (Glucosephosphates)
RN  - 0 (Palmitic Acids)
RN  - 0 (Phosphates)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 56-73-5 (Glucose-6-Phosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Cell Differentiation
MH  - Colonic Neoplasms/enzymology/pathology
MH  - Glucose-6-Phosphate
MH  - Glucosephosphates/pharmacology
MH  - Hexokinase/*metabolism
MH  - Humans
MH  - Magnesium/pharmacology
MH  - Mitochondria/enzymology
MH  - Palmitic Acid
MH  - Palmitic Acids/pharmacology
MH  - Phosphates/pharmacology
MH  - Solubility
MH  - Tumor Cells, Cultured/drug effects/*enzymology/pathology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1016/0020-711x(90)90146-t [doi]
PST - ppublish
SO  - Int J Biochem. 1990;22(4):419-23. doi: 10.1016/0020-711x(90)90146-t.