PMID- 23381818
OWN - NLM
STAT- MEDLINE
DCOM- 20140115
LR  - 20191027
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 28 Suppl 1
DP  - 2013
TI  - Effect of nitrofen in the final stages of development of the diaphragm muscle in 
      rats.
PG  - 13-8
LID - S0102-86502013001300004 [pii]
AB  - PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm 
      in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of 
      pregnant rats were divided into four groups: External Control (EC), composed of 
      non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of 
      nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational 
      day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo 
      (OO), composed of pregnant rats that received the oil on the same GD. The fetuses 
      were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body 
      weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and 
      subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle 
      were processed for histological staining with H/E and immunohistochemistry (IHQ) 
      for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA 
      compared to EC and OO groups (p < 0.001). HA was decreased on GD 18.5 compared to 
      21.5 (p < 0.001) and the HA/DA ratio showed no difference. IHQ showed decreased 
      expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen 
      model contributes to the understanding of muscularization in the formation of the 
      diaphragm where the myosin expression is decreased.
FAU - Goncalves, Frances Lilian Lanhellas
AU  - Goncalves FL
AD  - Division of Pediatric Surgery, Department of Surgery and Anatomy, School of 
      Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
FAU - Oliveira, Fabio Santana de
AU  - Oliveira FS
FAU - Schmidt, Augusto Frederico
AU  - Schmidt AF
FAU - Pereira, Luis Antonio Violin Dias
AU  - Pereira LA
FAU - Gallindo, Rodrigo Melo
AU  - Gallindo RM
FAU - Figueira, Rebeca Lopes
AU  - Figueira RL
FAU - Sbragia, Lourenco
AU  - Sbragia L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (Pesticides)
RN  - 0 (Phenyl Ethers)
RN  - EC 3.6.4.1 (Myosins)
RN  - N71UYG034A (nitrofen)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Hernia, Diaphragmatic/chemically induced/embryology/pathology
MH  - *Hernias, Diaphragmatic, Congenital
MH  - Immunohistochemistry
MH  - Myosins/*metabolism
MH  - Pesticides/*toxicity
MH  - Phenyl Ethers/*toxicity
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2013/02/13 06:00
MHDA- 2014/01/16 06:00
CRDT- 2013/02/06 06:00
PHST- 2013/02/06 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2014/01/16 06:00 [medline]
AID - S0102-86502013001300004 [pii]
AID - 10.1590/s0102-86502013001300004 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2013;28 Suppl 1:13-8. doi: 10.1590/s0102-86502013001300004.