PMID- 23382825
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20240318
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 1
DP  - 2013
TI  - Chaperone-usher fimbriae of Escherichia coli.
PG  - e52835
LID - 10.1371/journal.pone.0052835 [doi]
LID - e52835
AB  - Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many 
      Gram-negative bacteria. Escherichia coli genomes contain a large variety of 
      characterised and putative CU fimbrial operons, however, the classification and 
      annotation of individual loci remains problematic. Here we describe a 
      classification model based on usher phylogeny and genomic locus position to 
      categorise the CU fimbrial types of E. coli. Using the BLASTp algorithm, an 
      iterative usher protein search was performed to identify CU fimbrial operons from 
      35 E. coli (and one Escherichia fergusonnii) genomes representing different 
      pathogenic and phylogenic lineages, as well as 132 Escherichia spp. plasmids. A 
      total of 458 CU fimbrial operons were identified, which represent 38 distinct 
      fimbrial types based on genomic locus position and usher phylogeny. The majority 
      of fimbrial operon types occupied a specific locus position on the E. coli 
      chromosome; exceptions were associated with mobile genetic elements. A group of 
      core-associated E. coli CU fimbriae were defined and include the Type 1, Yad, 
      Yeh, Yfc, Mat, F9 and Ybg fimbriae. These genes were present as intact or 
      disrupted operons at the same genetic locus in almost all genomes examined. 
      Evaluation of the distribution and prevalence of CU fimbrial types among 
      different pathogenic and phylogenic groups provides an overview of group specific 
      fimbrial profiles and insight into the ancestry and evolution of CU fimbriae in 
      E. coli.
FAU - Wurpel, Daniel J
AU  - Wurpel DJ
AD  - Australian Infectious Diseases Research Centre, School of Chemistry and Molecular 
      Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
FAU - Beatson, Scott A
AU  - Beatson SA
FAU - Totsika, Makrina
AU  - Totsika M
FAU - Petty, Nicola K
AU  - Petty NK
FAU - Schembri, Mark A
AU  - Schembri MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130130
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (CfaC protein, E coli)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Escherichia coli/*genetics/metabolism
MH  - Escherichia coli Proteins/*genetics
MH  - Evolution, Molecular
MH  - Fimbriae, Bacterial/genetics/*metabolism
MH  - Genome, Bacterial
MH  - Molecular Chaperones/*genetics/metabolism
MH  - Phylogeny
PMC - PMC3559732
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/02/06 06:00
MHDA- 2013/09/05 06:00
PMCR- 2013/01/30
CRDT- 2013/02/06 06:00
PHST- 2012/08/31 00:00 [received]
PHST- 2012/11/22 00:00 [accepted]
PHST- 2013/02/06 06:00 [entrez]
PHST- 2013/02/06 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
PHST- 2013/01/30 00:00 [pmc-release]
AID - PONE-D-12-26484 [pii]
AID - 10.1371/journal.pone.0052835 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(1):e52835. doi: 10.1371/journal.pone.0052835. Epub 2013 Jan 30.