PMID- 23383483
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 10
DP  - 2012 Oct
TI  - [Rapid screening and confirmation of hormones in health foods by high performance 
      liquid chromatography-ion trap-time of flight tandem mass spectrometry].
PG  - 980-5
AB  - A method was developed for the screening, confirmation and quantification of 21 
      hormones including progesterones, etrogens, glucocorticoids and resorcylic acid 
      lactones in health foods by high performance liquid chromatography-ion trap-time 
      of flight tandem mass spectrometry (HPLC-IT-TOF-MS). The analytes in the sample 
      were extracted with acetonitrile containing 1.0% (v/v) acetic acid and the 
      extract was purified with the mixed QuEChERS sorbents. In the chromatographic 
      analysis, the 21 target compounds were separated on a C18 column with the 
      gradient elution using the mobile phases of acetonitrile and water containing 
      0.1% acetic acid. The mass analyzer was performed in positive and negative full 
      scan modes in one injection at the same time. The results showed that the linear 
      ranges of the 21 hormones were 5.0-250 microg/L with the correlation coefficients 
      above 0.993 and the limits of quantification (LOQ, S/N > or = 10) were 2.0-5.0 
      microg/kg and 1.0-2.5 microg/L for capsule and oral solution, respectively. The 
      method validation was carried out at three spiked levels, and the recoveries were 
      60.2%-116.0% with the relative standard deviations (RSDs) of 7.0%-18.3%. The 
      screening of analytes was performed by precision mass matching and library 
      searching. The secondary fragment ion spectra were employed to the confirmation. 
      This method is simple, fast, reliable, and can be applied to the simultaneous 
      screening and determination of hormones in health foods.
FAU - Wang, Meiling
AU  - Wang M
AD  - Inspection and Quarantine Technology Center, Hunan Entry-Exit Inspection and 
      Quarantine Bureau Changsha 410004, China.
FAU - Yan, Hongfei
AU  - Yan H
FAU - Fu, Shanliang
AU  - Fu S
FAU - Zhang, Fan
AU  - Zhang F
FAU - Yao, Jinting
AU  - Yao J
FAU - Dai, Hua
AU  - Dai H
FAU - Li, Yongjun
AU  - Li Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Estrogens)
RN  - 0 (Glucocorticoids)
RN  - 0 (Hormones)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Estrogens/analysis
MH  - Food Contamination/*analysis
MH  - Functional Food/*analysis
MH  - Glucocorticoids/analysis
MH  - Hormones/*analysis
MH  - Progesterone/analysis
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/02/07 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/02/07 06:00
PHST- 2013/02/07 06:00 [entrez]
PHST- 2013/02/07 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.08026 [doi]
PST - ppublish
SO  - Se Pu. 2012 Oct;30(10):980-5. doi: 10.3724/sp.j.1123.2012.08026.