PMID- 23383485
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 10
DP  - 2012 Oct
TI  - [Simultaneous identification and detection of 16 anabolic steroid hormones in 
      muscle using liquid chromatography oupled to quadrupole/linear ion trap mass 
      spectrometry].
PG  - 991-1001
AB  - A comprehensive method for simultaneous identification and detection of 16 
      anabolic steroid hormones (ASs, including andorgens, gestagens and their esters) 
      in muscle samples was developed with liquid chromatography coupled to 
      quadrupole/linear ion trap mass spectrometry (LC-Q/Trap-MS). The ASs in muscle 
      samples were extracted with acetonitrile under ultrasonic assistance. The extract 
      was defatted by n-hexane with liquid-liquid partitioning and followed by clean-up 
      with NH2 solid phase extraction (SPE) cartridge. The separation of analytes was 
      carried out on a CAPCELL PAK C18 MG II column (150 mm x 2.0 mm, 5.0 microm) using 
      mobile phases of 0.1% (v/v) formic acid in acetonitrile and 0.1% (v/v) formic 
      acid-5 mmol/L ammonium formate aqueous solution with gradient elution. A 
      scheduled multiple reaction monitoring (sMRM) in positive mode as survey scan and 
      an enhanced product ion (EPI) scan as dependent scan in an information-dependent 
      acquisition (IDA) experiment was adopted in mass spectrometry acquisition. 
      On-line lab-built MS/MS library and internal standards were employed for the 
      identification and quantification. As a result, the 16 ASs showed good linearity 
      with all correlation coefficients (r) no less than 0. 999 0 within the linear 
      ranges. The limits of quantification (LOQs, S/N > or = 10) for the 16 ASs were in 
      the range of 0.029-0.36 microg/kg. At the three spiked levels (0.5, 2.0 and 20 
      microg/kg), the overall recoveries ranged from 89.9% to 118% with the relative 
      standard deviations (RSDs) no more than 16.2% under within--laboratory 
      reproducibility conditions. The proposed method can be used to identify and 
      detect the 16 ASs in a single run, which makes it effective in residue 
      surveillance of anabolic hormones in muscle samples.
FAU - Zhang, Hongwei'
AU  - Zhang H
AD  - Technical Center of Entry-Exit Inspection and Qurantine, Shandong Entry-Exit 
      Inspection and Quarantine Bureau, Qingdao 266002, China. light04@126.com
FAU - Cai, Xue
AU  - Cai X
FAU - Lin, Liming
AU  - Lin L
FAU - Chen, Liangzhen
AU  - Chen L
FAU - Liang, Chengzhu
AU  - Liang C
FAU - Bao, Lei
AU  - Bao L
FAU - Tang, Zhixu
AU  - Tang Z
FAU - Niu, Zengyuan
AU  - Niu Z
FAU - Wang, Fengmei
AU  - Wang F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anabolic Agents)
RN  - 0 (Androgens)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Progestins)
SB  - IM
MH  - Anabolic Agents/analysis
MH  - Androgens/analysis
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Food Contamination/*analysis
MH  - Gonadal Steroid Hormones/*analysis
MH  - Muscle, Skeletal/*chemistry
MH  - Progestins/analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2013/02/07 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/02/07 06:00
PHST- 2013/02/07 06:00 [entrez]
PHST- 2013/02/07 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.08017 [doi]
PST - ppublish
SO  - Se Pu. 2012 Oct;30(10):991-1001. doi: 10.3724/sp.j.1123.2012.08017.