PMID- 23383493
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 10
DP  - 2012 Oct
TI  - [Development of a gas chromatography-mass spectrometry method for the metabolomic 
      study of rice (Oryza sativa L.) grain].
PG  - 1037-42
AB  - An analytical strategy for the metabolic profiling of rice grain was developed 
      based on gas chromatography-mass spectrometry (GC-MS). For the purpose of 
      obtaining abundant metabolite information, sample preparation step prior to 
      instrumental analysis is necessary to be optimized. D-optimal experimental design 
      was applied to optimize the extraction solvent. Four solvents, including water, 
      methanol, isopropanol and acetonitrile, and their combinations were evaluated for 
      the extraction efficiency using multivariate statistical analysis (partial least 
      square regression). The count of resolved peaks and the sum of peak areas were 
      taken as the evaluation indexes. Methanol/water (80:20, v/v) mixture was highly 
      efficient for rice metabolites and was selected as the suitable solvent 
      formulation. Then, the analytical characteristics of the method were measured. 
      More than 90% of the metabolites had satisfactory precisions, reproducibilities 
      and stabilities (relative standard deviations (RSDs) < 30%). Most of the detected 
      metabolites (about 88.0% of total peak area) showed good linear responses. With 
      the optimized analytical protocol, 315 metabolites were detected in rice and 86 
      of which were structurally identified by searching in the NIST 08/Wiley standard 
      mass spectral library, covering carbohydrates, amino acids, organic acids, 
      steroids and so on which showed a broad coverage of metabolite data. The 
      established method is expected to be useful for the metabolomic studies of rice.
FAU - Zhou, Jia
AU  - Zhou J
AD  - (CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Chinese Academy of Sciences, Dalian 116023, China.
FAU - Wang, Shuangyuan
AU  - Wang S
FAU - Chang, Yuwei
AU  - Chang Y
FAU - Zhao, Yanni
AU  - Zhao Y
FAU - Lu, Xin
AU  - Lu X
FAU - Zhao, Chunxia
AU  - Zhao C
FAU - Xu, Guowang
AU  - Xu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amino Acids)
RN  - 0 (Carbohydrates)
RN  - 0 (Steroids)
SB  - IM
MH  - Amino Acids/analysis
MH  - Carbohydrates/analysis
MH  - Edible Grain/*metabolism
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Metabolomics/*methods
MH  - Oryza/*metabolism
MH  - Steroids/analysis
EDAT- 2013/02/07 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/02/07 06:00
PHST- 2013/02/07 06:00 [entrez]
PHST- 2013/02/07 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.06042 [doi]
PST - ppublish
SO  - Se Pu. 2012 Oct;30(10):1037-42. doi: 10.3724/sp.j.1123.2012.06042.