PMID- 23384907
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20130206
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 33
IP  - 10
DP  - 2012 Oct
TI  - [Study on the role of fluorescence in situ hybridization in cytogenetic 
      evaluation of myelodysplastic syndrome].
PG  - 839-42
AB  - OBJECTIVE: To exploit the role of bone marrow (BM) and peripheral blood (PB) 
      fluorescence in situ hybridization (FISH) in cytogenetic evaluation of 
      myelodysplastic syndrome (MDS). METHODS: The metaphase cytogenetics and BM 
      interphase FISH were prospectively compared in 112 cases of de novo MDS. At the 
      same time, comparison of BM and PB FISH was conducted in 56 cases. RESULTS: The 
      differences between metaphase cytogenetics and BM FISH were observed in 22 (54%) 
      of 41 cases with clonal karyotypic abnormalities, most of differences were caused 
      by the limitation of FISH probe panel which could not target all of the regions 
      with aberrations. Only 6 (27%) of 22 differences were involved in our probe 
      regions, the FISH results did not change their cytogenetic risk categories. BM 
      FISH testing was abnormal in 15 (21%) of 71 cases with normal karyotypes, FISH 
      testing was abnormal in 14/51 (27%) and 1/20 (5%) cases with fewer than 20 normal 
      metaphases or more than 20 normal metaphases. Comparison of FISH results of PB 
      and BM samples showed abnormal PB FISH results in 21 (72%) of 29 cases with 
      abnormal BM FISH results, and in 1 (4%) of 27 cases with normal BM FISH results. 
      CONCLUSION: BM FISH should be used to MDS cases with fewer than 20 normal 
      metaphases. Although PB FISH testing is limited by a relatively high false 
      negative rate, it is a reasonable choice to cases with failure of BM aspiration.
FAU - Qu, Shi-qiang
AU  - Qu SQ
AD  - Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin, China.
FAU - Xu, Ze-feng
AU  - Xu ZF
FAU - Li, Cheng-wen
AU  - Li CW
FAU - Liu, Xu-ping
AU  - Liu XP
FAU - Qin, Tie-jun
AU  - Qin TJ
FAU - Zhang, Yue
AU  - Zhang Y
FAU - Xiao, Zhi-jian
AU  - Xiao ZJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/blood/*genetics
MH  - Prospective Studies
MH  - Young Adult
EDAT- 2013/02/07 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/02/07 06:00
PHST- 2013/02/07 06:00 [entrez]
PHST- 2013/02/07 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2012 Oct;33(10):839-42.