PMID- 23386101 OWN - NLM STAT- MEDLINE DCOM- 20140211 LR - 20211021 IS - 1348-4400 (Electronic) IS - 0916-8818 (Print) IS - 0916-8818 (Linking) VI - 59 IP - 3 DP - 2013 TI - Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum. PG - 219-24 AB - Luteoprotective mechanisms of luteinizing hormone (LH) involved in the maintenance of bovine corpus luteum (CL) function have not been completely clarified. Since antioxidant enzymes are well documented as antiapoptotic factors in the CL of many mammals, we hypothesized that the luteoprotective action of LH is mediated by stimulating the local production and action of antioxidant enzymes. To test the above hypothesis, in the present study, we examined the mechanisms involved in the luteoprotective actions of LH. Cultured bovine luteal cells obtained from the CL at the mid-luteal stage (days 8-12 of the estrous cycle) were treated with LH (10 ng/ml), onapristone (OP; a specific progesterone receptor antagonist, 100 muM) and diethyldithiocarbamate [DETC; an inhibitor of superoxide dismutase (SOD), 100 muM] for 24 h. LH in combination with or without OP significantly increased the mRNA and protein expressions of manganese SOD (Mn-SOD) and catalase (CATA) and SOD activity. While LH alone significantly increased the mRNA and protein expressions of SOD containing copper and zinc (Cu,Zn-SOD), OP in combination with or without LH significantly decreased the mRNA and protein expressions of Cu,Zn-SOD. In addition, Cu,Zn-SOD, Mn-SOD and CATA mRNA expressions were higher at the mid luteal phase than the other luteal phases. LH in combination with DETC significantly decreased LH-increased cell viability. The overall results suggest that LH increases cell viability by LH-increased antioxidant enzymes, resulting in maintenance of CL function during the luteal phase in cattle. FAU - Kawaguchi, Syota AU - Kawaguchi S AD - Laboratory of Reproductive Physiology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. FAU - Sakumoto, Ryosuke AU - Sakumoto R FAU - Okuda, Kiyoshi AU - Okuda K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130204 PL - Japan TA - J Reprod Dev JT - The Journal of reproduction and development JID - 9438792 RN - 0 (Antioxidants) RN - 0 (RNA, Messenger) RN - 0 (Reactive Oxygen Species) RN - 0 (Receptors, Progesterone) RN - 4G7DS2Q64Y (Progesterone) RN - 9002-67-9 (Luteinizing Hormone) RN - EC 1.11.1.6 (Catalase) RN - EC 1.15.1.1 (Superoxide Dismutase) SB - IM MH - Animals MH - Antioxidants/*metabolism MH - Apoptosis MH - Catalase/metabolism MH - Cattle MH - Cell Survival MH - Corpus Luteum/*metabolism MH - Female MH - *Gene Expression Regulation, Enzymologic MH - Luteal Phase MH - Luteinizing Hormone/*metabolism MH - Progesterone/metabolism MH - RNA, Messenger/metabolism MH - Reactive Oxygen Species/*metabolism MH - Receptors, Progesterone/metabolism MH - Superoxide Dismutase/metabolism MH - Time Factors PMC - PMC3934140 EDAT- 2013/02/07 06:00 MHDA- 2014/02/12 06:00 PMCR- 2013/06/01 CRDT- 2013/02/07 06:00 PHST- 2013/02/07 06:00 [entrez] PHST- 2013/02/07 06:00 [pubmed] PHST- 2014/02/12 06:00 [medline] PHST- 2013/06/01 00:00 [pmc-release] AID - DN/JST.JSTAGE/jrd/2012-186 [pii] AID - 2012-186 [pii] AID - 10.1262/jrd.2012-186 [doi] PST - ppublish SO - J Reprod Dev. 2013;59(3):219-24. doi: 10.1262/jrd.2012-186. Epub 2013 Feb 4.