PMID- 23388332
OWN - NLM
STAT- MEDLINE
DCOM- 20140117
LR  - 20181202
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 29
IP  - 2
DP  - 2013 Feb
TI  - [Role of INGAP-pp in the differentiation of hUCMSCs into insulin producing 
      cells].
PG  - 141-5
AB  - OBJECTIVE: To find out a protocol for the efficient and directional 
      differentiation from human umbilical cord mesenchymal stem cells (hUCMSCs) into 
      insulin-producing cells (IPCs). METHODS: HUCMSCs were induced to differentiate 
      into IPCs by an improved protocol based on a traditional protocol with addition 
      of islet neogenesis-associated protein-pp (INGAP-pp), and the IPCs were 
      identified by morphological observation, flow cytometry, real-time quantitative 
      PCR, immunofluorescence and ELISA. RESULTS: Compared with the traditional 
      protocol, the improved protocol resulted in the higher rate of C peptide positive 
      cells (P<0.05) and the increased mRNA expressions of pancreas development related 
      transcription factor PDX-1, Ngn3, NeuroD1, MafA, insulin, Glut-2 (P<0.05). 
      Meanwhile, by the improved protocol the protein expressions of C peptide, PDX-1 
      and Nkx6.1 were positive, and insulin and C-peptide release were promoted 
      (P<0.05) as demonstrated by glucose challenge test. CONCLUSION: INGAP-pp may 
      facilitate the differentiation of hUCMSCs into IPCs. However, the IPCs are not as 
      mature as normal human islet beta cells.
FAU - Chen, Tao
AU  - Chen T
AD  - Department of Rheumatism, Jinan University, Shenzhen, China. s_tchen32163.com
FAU - Wang, Yunshuai
AU  - Wang Y
FAU - Gu, Lehui
AU  - Gu L
FAU - Zhou, Shuyan
AU  - Zhou S
FAU - Ye, Jian
AU  - Ye J
FAU - Jian, Youqiang
AU  - Jian Y
FAU - Li, Furong
AU  - Li F
FAU - Qi, Hui
AU  - Qi H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Pancreatitis-Associated Proteins)
RN  - 0 (REG3A protein, human)
SB  - IM
MH  - Antigens, Neoplasm/*metabolism
MH  - Biomarkers, Tumor/*metabolism
MH  - *Cell Differentiation
MH  - Humans
MH  - Insulin-Secreting Cells/*cytology/*metabolism
MH  - Lectins, C-Type/*metabolism
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Pancreatitis-Associated Proteins
EDAT- 2013/02/08 06:00
MHDA- 2014/01/18 06:00
CRDT- 2013/02/08 06:00
PHST- 2013/02/08 06:00 [entrez]
PHST- 2013/02/08 06:00 [pubmed]
PHST- 2014/01/18 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Feb;29(2):141-5.