PMID- 23388354
OWN - NLM
STAT- MEDLINE
DCOM- 20130711
LR  - 20181202
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1831
IP  - 6
DP  - 2013 Jun
TI  - Characterization of palmitoylation of ATP binding cassette transporter G1: effect 
      on protein trafficking and function.
PG  - 1067-78
LID - S1388-1981(13)00038-3 [pii]
LID - 10.1016/j.bbalip.2013.01.019 [doi]
AB  - ATP-binding cassette transporter G1 (ABCG1) mediates cholesterol efflux onto 
      lipidated apolipoprotein A-I and HDL and plays a role in various important 
      physiological functions. However, the mechanism by which ABCG1 mediates 
      cholesterol translocation is unclear. Protein palmitoylation regulates many 
      functions of proteins such as ABCA1. Here we investigated if ABCG1 is 
      palmitoylated and the subsequent effects on ABCG1-mediated cholesterol efflux. We 
      demonstrated that ABCG1 is palmitoylated in both human embryonic kidney 293 cells 
      and in mouse macrophage, J774. Five cysteine residues located at positions 26, 
      150, 311, 390 and 402 in the NH2-terminal cytoplasmic region of ABCG1 were 
      palmitoylated. Removal of palmitoylation at Cys311 by mutating the residue to Ala 
      (C311A) or Ser significantly decreased ABCG1-mediated cholesterol efflux. On the 
      other hand, removal of palmitoylation at sites 26, 150, 390 and 402 had no 
      significant effect. We further demonstrated that mutations of Cys311 affected 
      ABCG1 trafficking from the endoplasmic reticulum. Therefore, our data suggest 
      that palmitoylation plays a critical role in ABCG1-mediated cholesterol efflux 
      through the regulation of trafficking.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Gu, Hong-mei
AU  - Gu HM
AD  - Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, 
      Canada.
FAU - Li, Ge
AU  - Li G
FAU - Gao, Xia
AU  - Gao X
FAU - Berthiaume, Luc G
AU  - Berthiaume LG
FAU - Zhang, Da-wei
AU  - Zhang DW
LA  - eng
GR  - MOP93794/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130204
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Lipoproteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Cholesterol/metabolism
MH  - Cysteine/chemistry/genetics/metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Kidney/cytology/*metabolism
MH  - Lipoproteins/genetics/*metabolism
MH  - *Lipoylation
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Mutation/genetics
MH  - *Protein Processing, Post-Translational
MH  - Protein Transport
EDAT- 2013/02/08 06:00
MHDA- 2013/07/13 06:00
CRDT- 2013/02/08 06:00
PHST- 2012/11/08 00:00 [received]
PHST- 2013/01/14 00:00 [revised]
PHST- 2013/01/25 00:00 [accepted]
PHST- 2013/02/08 06:00 [entrez]
PHST- 2013/02/08 06:00 [pubmed]
PHST- 2013/07/13 06:00 [medline]
AID - S1388-1981(13)00038-3 [pii]
AID - 10.1016/j.bbalip.2013.01.019 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2013 Jun;1831(6):1067-78. doi: 
      10.1016/j.bbalip.2013.01.019. Epub 2013 Feb 4.