PMID- 23388415
OWN - NLM
STAT- MEDLINE
DCOM- 20131107
LR  - 20211203
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 110
IP  - 6
DP  - 2013 Sep 28
TI  - Post-exercise whey protein hydrolysate supplementation induces a greater increase 
      in muscle protein synthesis than its constituent amino acid content.
PG  - 981-7
LID - 10.1017/S0007114512006174 [doi]
AB  - It is well known that ingestion of a protein source is effective in stimulating 
      muscle protein synthesis after exercise. In addition, there are numerous reports 
      on the impact of leucine and leucine-rich whey protein on muscle protein 
      synthesis and mammalian target of rapamycin (mTOR) signalling. However, there is 
      only limited information on the effects of whey protein hydrolysates (WPH) on 
      muscle protein synthesis and mTOR signalling. The aim of the present study was to 
      compare the effects of WPH and amino acids on muscle protein synthesis and the 
      initiation of translation in skeletal muscle during the post-exercise phase. Male 
      Sprague-Dawley rats swam for 2 h to depress muscle protein synthesis. Immediately 
      after exercise, the animals were administered either carbohydrate (CHO), CHO plus 
      an amino acid mixture (AA) or CHO plus WPH. At 1 h after exercise, the 
      supplements containing whey-based protein (AA and WPH) caused a significant 
      increase in the fractional rate of protein synthesis (FSR) compared with CHO. WPH 
      also caused a significant increase in FSR compared with AA. Post-exercise 
      ingestion of WPH caused a significant increase in the phosphorylation of mTOR 
      levels compared with AA or CHO. In addition, WPH caused greater phosphorylation 
      of ribosomal protein S6 kinase and eukaryotic initiation factor 4E-binding 
      protein 1 than AA and CHO. In contrast, there was no difference in plasma amino 
      acid levels following supplementation with either AA or WPH. These results 
      indicate that WPH may include active components that are superior to amino acids 
      for stimulating muscle protein synthesis and initiating translation.
FAU - Kanda, Atsushi
AU  - Kanda A
AD  - Food Science Research Laboratories, Meiji Company Limited, 540 Naruda, Odawara, 
      Kanagawa 250-0862, Japan.
FAU - Nakayama, Kyosuke
AU  - Nakayama K
FAU - Fukasawa, Tomoyuki
AU  - Fukasawa T
FAU - Koga, Jinichiro
AU  - Koga J
FAU - Kanegae, Minoru
AU  - Kanegae M
FAU - Kawanaka, Kentaro
AU  - Kawanaka K
FAU - Higuchi, Mitsuru
AU  - Higuchi M
LA  - eng
PT  - Journal Article
DEP - 20130207
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Amino Acids)
RN  - 0 (Carrier Proteins)
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Insulin)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Milk Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Whey Proteins)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acids/blood/*chemistry/pharmacology
MH  - Animals
MH  - Carrier Proteins/genetics/metabolism
MH  - Dietary Supplements
MH  - Gene Expression Regulation/drug effects
MH  - Insulin/blood
MH  - Intracellular Signaling Peptides and Proteins
MH  - Male
MH  - Milk Proteins/*administration & dosage/*pharmacology
MH  - Muscle Proteins/genetics/*metabolism
MH  - Muscle, Skeletal/*physiology
MH  - Phosphoproteins/genetics/metabolism
MH  - Phosphorylation
MH  - Physical Conditioning, Animal/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6 Kinases/genetics/metabolism
MH  - Swimming/physiology
MH  - TOR Serine-Threonine Kinases
MH  - Whey Proteins
EDAT- 2013/02/08 06:00
MHDA- 2013/11/08 06:00
CRDT- 2013/02/08 06:00
PHST- 2013/02/08 06:00 [entrez]
PHST- 2013/02/08 06:00 [pubmed]
PHST- 2013/11/08 06:00 [medline]
AID - S0007114512006174 [pii]
AID - 10.1017/S0007114512006174 [doi]
PST - ppublish
SO  - Br J Nutr. 2013 Sep 28;110(6):981-7. doi: 10.1017/S0007114512006174. Epub 2013 
      Feb 7.