PMID- 23389678 OWN - NLM STAT- MEDLINE DCOM- 20131017 LR - 20131121 IS - 1753-9455 (Electronic) IS - 1753-9447 (Linking) VI - 7 IP - 2 DP - 2013 Apr TI - Effect of acute exercise on cardiovascular hemodynamic and red blood cell concentrations of purine nucleotides in hypertensive compared with normotensives rats. PG - 63-74 LID - 10.1177/1753944712470297 [doi] AB - OBJECTIVE: The mechanisms of exercise-induced health benefits are complex and not fully understood. This study investigated the effects of exercise and hypertension on cardiovascular hemodynamic responses and red blood cell (RBC) concentrations of purine nucleotides using normotensive and hypertensive rat models in vivo. METHODS: Sprague Dawley rats (SDRs) and spontaneously hypertensive rats (SHRs) were exercised on a treadmill for 15 min at a speed of 10 m/min and 5% grade. Blood samples were obtained from each rat before, during, and after exercise for measurement of adenosine 5'-triphosphate (ATP) and guanosine 5'-triphosphate (GTP) concentrations in RBCs by a validated high-performance liquid chromatography assay. They were returned to a restrainer after exercise, and hemodynamic recording collected continuously up to 6 h. Two separate groups (SDRs and SHRs) without exercise were used as controls. Biomarker data were compared between SDRs and SHRs using analysis of variance and t test and difference considered significant at p < 0.05. RESULTS: The study has demonstrated for the first time a difference in the postexercise effect between SDRs and SHRs. The 15 min of exercise significantly increased systolic blood pressure (SBP) (129 +/- 16 to 162 +/- 26 mmHg) and heart rate (HR) (416 +/- 29 to 491 +/- 26 bpm) in SDRs (p < 0.05), but not in SHRs. The postexercise hemodynamic effects were more profound in SHRs. SBP and diastolic blood pressure (DBP) also fell significantly in the control group of SHRs (SBP 184 +/- 14 to 152 +/- 29 mmHg and DBP 149 +/- 9 to 120 +/- 14 mmHg, p < 0.05 for both) towards the end of the experiment but not in the SDR group. The RBC concentrations of ATP and GTP increased after exercise in both SDRs and SHRs which were significantly correlated with the postexercise hemodynamic effect (p < 0.05). CONCLUSION: SHRs were more tolerant to increases in HR and SBP induced by exercise, and have more profound postexercise hemodynamic effects than SDRs. The hemodynamic effects were linked closely with RBC concentrations of ATP and GTP in both SDRs and SHRs. FAU - Yeung, Pollen K F AU - Yeung PK AD - College of Pharmacy, Dalhousie University, Halifax, NS, Canada. pollen.yeung@dal.ca FAU - Dauphinee, Julie AU - Dauphinee J FAU - Marcoux, Tanya AU - Marcoux T LA - eng GR - ROP86932/Canadian Institutes of Health Research/Canada PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130206 PL - England TA - Ther Adv Cardiovasc Dis JT - Therapeutic advances in cardiovascular disease JID - 101316343 RN - 86-01-1 (Guanosine Triphosphate) RN - 8L70Q75FXE (Adenosine Triphosphate) SB - IM MH - Adaptation, Physiological MH - Adenosine Triphosphate/*blood MH - Animals MH - Blood Pressure MH - Chromatography, High Pressure Liquid MH - Disease Models, Animal MH - Erythrocytes/*metabolism MH - Exercise Test MH - Guanosine Triphosphate/*blood MH - Heart Rate MH - *Hemodynamics MH - Hypertension/*blood/*physiopathology MH - Male MH - *Physical Exertion MH - Rats MH - Rats, Inbred SHR MH - Rats, Sprague-Dawley MH - Time Factors MH - Up-Regulation EDAT- 2013/02/08 06:00 MHDA- 2013/10/18 06:00 CRDT- 2013/02/08 06:00 PHST- 2013/02/08 06:00 [entrez] PHST- 2013/02/08 06:00 [pubmed] PHST- 2013/10/18 06:00 [medline] AID - 1753944712470297 [pii] AID - 10.1177/1753944712470297 [doi] PST - ppublish SO - Ther Adv Cardiovasc Dis. 2013 Apr;7(2):63-74. doi: 10.1177/1753944712470297. Epub 2013 Feb 6.