PMID- 23390747
OWN - NLM
STAT- MEDLINE
DCOM- 20130318
LR  - 20191112
IS  - 1505-1773 (Print)
IS  - 1505-1773 (Linking)
VI  - 15
IP  - 4
DP  - 2012
TI  - Blood dendritic cells in cattle infected with bovine leukemia virus (BLV): 
      isolation and phenotyping.
PG  - 599-608
AB  - Dendritic cells (DCs) are most potent antigen presenting cells (APCs) with unique 
      ability to prime effective immune responses. They express higher levels of MHC 
      class II and accesory molecules on their surface, than other professional APCs. 
      The investigations were performed on DCs generated from blood with the use of 
      microbeads magnetically labeled with mouse anti human CD14. Flow cytometry was 
      applied for determination of DCs immunophenotype in healthy and naturally 
      infected with BLV cattle. For immunophenotyping mouse monoclonal antibodies anti 
      bovine: CD11a, CD11b, CD11c, MHC-I and MHC-II were used. Our results demonstrated 
      that dendritic cells infected with BLV expressed very high percentage of 
      determinants: CD11a, CD11b, CD11c, MHC-I and MHC-II class. Leukaemic DCs 
      exhibited DCs morphology and had a phenotype of mature DCs. The expression of 
      gp51 glycoprotein of BLV on leukaemic DCs was detected in flow cytometry 
      investigations.
FAU - Szczotka, M
AU  - Szczotka M
AD  - Department of Biochemistry, National Veterinary Research Institute, Al. 
      Partyzantow 57, 24-100 Pulawy, Poland. szczotka@piwet.pulawy.pl
FAU - Kuzmak, J
AU  - Kuzmak J
FAU - Kostro, K
AU  - Kostro K
FAU - Bednarek, D
AU  - Bednarek D
FAU - Purzycka, M
AU  - Purzycka M
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pol J Vet Sci
JT  - Polish journal of veterinary sciences
JID - 101125473
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/genetics/metabolism
MH  - Cattle
MH  - Dendritic Cells/*physiology
MH  - Enzootic Bovine Leukosis/*blood
MH  - Gene Expression Regulation/immunology/physiology
MH  - Leukemia Virus, Bovine/*metabolism
MH  - Viral Proteins/genetics/metabolism
EDAT- 2013/02/09 06:00
MHDA- 2013/03/19 06:00
CRDT- 2013/02/09 06:00
PHST- 2013/02/09 06:00 [entrez]
PHST- 2013/02/09 06:00 [pubmed]
PHST- 2013/03/19 06:00 [medline]
AID - 10.2478/v10181-012-0095-2 [doi]
PST - ppublish
SO  - Pol J Vet Sci. 2012;15(4):599-608. doi: 10.2478/v10181-012-0095-2.