PMID- 23391825
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR  - 20130325
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 189
IP  - 1
DP  - 2013 Apr
TI  - Calibrated real-time polymerase chain reaction for specific quantitation of 
      HHV-6A and HHV-6B in clinical samples.
PG  - 172-9
LID - S0166-0934(13)00023-2 [pii]
LID - 10.1016/j.jviromet.2013.01.018 [doi]
AB  - The recent classification of human herpesvirus 6 (HHV-6) A and B, previously 
      considered as two variants of the same virus, as two distinct herpesvirus 
      species, emphasizes the need to develop and standardize specific methods for 
      their detection and quantitation for clinical use. The development of two highly 
      sensitive calibrated real-time PCR to quantify HHV-6A and -6B variants in 
      clinical specimen is described. Both assays displayed the same wide linear 
      dynamic range from 10(0) to 10(6) copies of viral DNA in a single reaction and 
      sensitivity of one copy/reaction. These systems allow for HHV-6A/B DNA load 
      quantitation in different types of clinical specimens: blood or tissue cells when 
      combined with the CCR5 assay; cell-free samples (plasma or other biological 
      fluids) in combination with the calibrator technology. Due to the absence of 
      cross-amplification and cross-hybridization, these methods detect minute amounts 
      of one viral species even in the presence of a large excess of the other, 
      allowing a specific quantitation of both viruses in the case of mixed infections. 
      The new qPCR methods provide sensitive and specific tool for monitoring HHV-6A/B 
      DNA load in clinical samples, facilitating the study of these viruses in human 
      diseases.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Cassina, Giulia
AU  - Cassina G
AD  - Unit of Human Virology Division of Immunology, Transplantation and Infectious 
      Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, 
      Italy.
FAU - Russo, Domenico
AU  - Russo D
FAU - De Battista, Davide
AU  - De Battista D
FAU - Broccolo, Francesco
AU  - Broccolo F
FAU - Lusso, Paolo
AU  - Lusso P
FAU - Malnati, Mauro S
AU  - Malnati MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130204
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA, Viral/*analysis/genetics
MH  - Herpesvirus 6, Human/*classification/genetics/*isolation & purification
MH  - Humans
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Roseolovirus Infections/*diagnosis
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Viral Load
EDAT- 2013/02/09 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/02/09 06:00
PHST- 2011/12/05 00:00 [received]
PHST- 2013/01/23 00:00 [revised]
PHST- 2013/01/28 00:00 [accepted]
PHST- 2013/02/09 06:00 [entrez]
PHST- 2013/02/09 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - S0166-0934(13)00023-2 [pii]
AID - 10.1016/j.jviromet.2013.01.018 [doi]
PST - ppublish
SO  - J Virol Methods. 2013 Apr;189(1):172-9. doi: 10.1016/j.jviromet.2013.01.018. Epub 
      2013 Feb 4.