PMID- 23391931 OWN - NLM STAT- MEDLINE DCOM- 20130814 LR - 20130226 IS - 1347-6947 (Electronic) IS - 0916-8451 (Linking) VI - 77 IP - 2 DP - 2013 TI - Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe. PG - 405-8 AB - White rot fungus, Phanerochaete chrysosporium, and brown rot fungus, Postia placenta, grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. Mycelia grown on wood chips were also clearly detected by PNA-FISH following blocking treatment. To the best of our knowledge, this is the first report on the visualization of fungi in wood by FISH. FAU - Nakada, Yuji AU - Nakada Y AD - Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan. FAU - Nakaba, Satoshi AU - Nakaba S FAU - Matsunaga, Hiroshi AU - Matsunaga H FAU - Funada, Ryo AU - Funada R FAU - Yoshida, Makoto AU - Yoshida M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130207 PL - England TA - Biosci Biotechnol Biochem JT - Bioscience, biotechnology, and biochemistry JID - 9205717 RN - 0 (Nucleic Acid Probes) RN - 0 (Peptide Nucleic Acids) RN - 0 (RNA, Ribosomal, 18S) SB - IM MH - In Situ Hybridization, Fluorescence/*methods MH - Microscopy, Fluorescence MH - Mycelium/growth & development/*ultrastructure MH - Nucleic Acid Probes/*chemistry MH - Peptide Nucleic Acids/*chemistry MH - Phanerochaete/growth & development/*ultrastructure MH - Polyporales/growth & development/*ultrastructure MH - RNA, Ribosomal, 18S/analysis MH - Wood/microbiology EDAT- 2013/02/09 06:00 MHDA- 2013/08/15 06:00 CRDT- 2013/02/09 06:00 PHST- 2013/02/09 06:00 [entrez] PHST- 2013/02/09 06:00 [pubmed] PHST- 2013/08/15 06:00 [medline] AID - DN/JST.JSTAGE/bbb/120777 [pii] AID - 10.1271/bbb.120777 [doi] PST - ppublish SO - Biosci Biotechnol Biochem. 2013;77(2):405-8. doi: 10.1271/bbb.120777. Epub 2013 Feb 7.