PMID- 23394223
OWN - NLM
STAT- MEDLINE
DCOM- 20140123
LR  - 20220311
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 133
IP  - 6
DP  - 2013 Jun
TI  - Expression of HMGA1 and ezrin in laryngeal squamous cell carcinoma.
PG  - 626-32
LID - 10.3109/00016489.2012.758388 [doi]
AB  - CONCLUSION: The overexpression of HMGA1 or Ezrin may contribute to the 
      carcinogenesis, development, and metastasis of laryngeal squamous cell carcinoma 
      (LSCC). OBJECTIVE: To investigate the expression of HMGA1 and Ezrin in LSCC and 
      analyze their clinical significance. METHODS: The expression of HMGA1 and Ezrin 
      was analyzed by immunohistochemistry (IHC) in 50 cases of LSCC. Thirty cases of 
      laryngeal polyp and 30 cases of atypical hyperplasia of larynx were studied as 
      controls. The expression of HMGA1 and Ezrin was analyzed by real-time PCR and by 
      Western blot in 30 cases of LSCC; samples from adjacent normal epithelial tissues 
      in 30 cases were studied as controls. RESULTS: (1) IHC revealed that the positive 
      rate of HMGA1 protein was 68.0% (34/50), 53.3% (16/30), and 13. 3% (4/30) in 
      LSCC, atypical hyperplasia of larynx, and laryngeal polyp (p < 0.05), and the 
      positive rate of Ezrin protein was 64.0% (32/50), 50.0% (15/30), and 23.3% (7/30) 
      (p < 0.01), respectively. (2) Real-time PCR demonstrated that the mean relative 
      mRNA expression levels of HMGA1 in LSCC and in normal tissues were 2.41 +/- 0.40 
      and 1.05 +/- 0.18, respectively (p < 0.01). The mRNA levels of Ezrin in LSCC and in 
      normal tissues were 1.79 +/- 0.27 and 1.04 +/- 0.22, respectively (p < 0.05). (3) 
      Western blotting revealed that the mean relative protein expression levels of 
      HMGA1 in LSCC and in normal tissues were 1.73 +/- 0.60 and 0.35 +/- 0.17, 
      respectively (p < 0.01). The protein levels of Ezrin in LSCC and in normal 
      tissues were 1.82 +/- 0.77 and 0.42 +/- 0.20, respectively (p < 0.01).
FAU - Wang, De-Sheng
AU  - Wang DS
AD  - Department of Otolaryngology, Affiliated Union Hospital of Fujian Medical 
      University, Fujian, Fuzhou, China. fzwds@yahoo.com
FAU - Pan, Chun-Chen
AU  - Pan CC
FAU - Lai, Hai-Chun
AU  - Lai HC
FAU - Huang, Jian-Min
AU  - Huang JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130211
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (HMGA Proteins)
RN  - 0 (ezrin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/biosynthesis/*genetics
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cytoskeletal Proteins/biosynthesis/*genetics
MH  - Female
MH  - HMGA Proteins/biosynthesis/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Real-Time Polymerase Chain Reaction
MH  - Respiratory Mucosa/metabolism
EDAT- 2013/02/12 06:00
MHDA- 2014/01/24 06:00
CRDT- 2013/02/12 06:00
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2014/01/24 06:00 [medline]
AID - 10.3109/00016489.2012.758388 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2013 Jun;133(6):626-32. doi: 10.3109/00016489.2012.758388. Epub 
      2013 Feb 11.