PMID- 23394777
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR  - 20130318
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 99
IP  - 4
DP  - 2013 Mar 15
TI  - Use of array comparative genomic hybridization (array-CGH) for embryo assessment: 
      clinical results.
PG  - 1044-8
LID - S0015-0282(13)00137-4 [pii]
LID - 10.1016/j.fertnstert.2013.01.094 [doi]
AB  - OBJECTIVE: To review clinical outcomes after preimplantation genetic screening. 
      Most methods of embryo viability assessment involve morphologic evaluation at 
      different preimplantation developmental stages. A weak association between 
      blastocyst morphology and aneuploidy has been described, supporting the basis for 
      preimplantation genetic screening (PGS) for assessment of embryo viability. The 
      expected improvement in reproductive outcome rates has been reached with the 
      application of microarrays based on comparative genomic hybridization (CGH) in 
      clinical routine PGS. DESIGN: Review of published studies and own unpublished 
      data. SETTING: University-affiliated private institution. PATIENT(S): IVF 
      patients undergoing PGS at different stages. INTERVENTION(S): PGS with polar 
      body, cleavage-stage, and blastocyst biopsies. MAIN OUTCOME MEASURE(S): 
      Aneuploidy, implantation, and pregnancy rates. RESULTS: The clinical outcome 
      after analysis of all 24 chromosomes improved pregnancy and implantation rates 
      for different indications to a higher degree than the previously available 
      technology, fluorescence in situ hybridization (FISH), in which only a limited 
      number of chromosomes could be analyzed. CONCLUSION(S): Most of the data 
      regarding the controversy of day-3 biopsy come from FISH cycles, and the utility 
      of day-3 biopsy with new array-CGH technology should be further evaluated through 
      randomized controlled trials. The current trend is blastocyst biopsy with a fresh 
      transfer or vitrification for transfer in a nonstimulated cycle.
CI  - Copyright (c) 2013 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Rubio, Carmen
AU  - Rubio C
AD  - Instituto Valenciano de Infertilidad, Instituto Universitario IVI, Valencia 
      University, Incliva, Valencia, Spain. carmen.rubio@iviomics.com
FAU - Rodrigo, Lorena
AU  - Rodrigo L
FAU - Mir, Pere
AU  - Mir P
FAU - Mateu, Emilia
AU  - Mateu E
FAU - Peinado, Vanessa
AU  - Peinado V
FAU - Milan, Miguel
AU  - Milan M
FAU - Al-Asmar, Nasser
AU  - Al-Asmar N
FAU - Campos-Galindo, Inmaculada
AU  - Campos-Galindo I
FAU - Garcia, Sandra
AU  - Garcia S
FAU - Simon, Carlos
AU  - Simon C
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20130205
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Blastocyst/*physiology
MH  - Comparative Genomic Hybridization/*methods
MH  - Embryo Transfer/methods
MH  - Female
MH  - Fertilization in Vitro/*methods
MH  - Humans
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
EDAT- 2013/02/12 06:00
MHDA- 2013/05/08 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/08/31 00:00 [received]
PHST- 2013/01/08 00:00 [revised]
PHST- 2013/01/09 00:00 [accepted]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
AID - S0015-0282(13)00137-4 [pii]
AID - 10.1016/j.fertnstert.2013.01.094 [doi]
PST - ppublish
SO  - Fertil Steril. 2013 Mar 15;99(4):1044-8. doi: 10.1016/j.fertnstert.2013.01.094. 
      Epub 2013 Feb 5.