PMID- 23394901
OWN - NLM
STAT- MEDLINE
DCOM- 20130827
LR  - 20130311
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 61
IP  - 3
DP  - 2013 Mar
TI  - RelB/p50 regulates TNF production in LPS-stimulated dendritic cells and 
      macrophages.
PG  - 736-40
LID - S1043-4666(13)00027-6 [pii]
LID - 10.1016/j.cyto.2012.12.029 [doi]
AB  - Dendritic cells (DCs) and macrophages are effective antigen-presenting cells, and 
      DCs, once matured, have the ability to potently activate naive T cells. While the 
      canonical p65/p50 NF-kappaB pathway seems to have an important role during 
      LPS-stimulation of these cells, the specific contribution of the non-canonical 
      RelB/p50 subunits is not clear yet. We aimed to investigate the relevance of this 
      pathway in DCs and macrophages by using replication-deficient adenoviruses 
      overexpressing RelB and p50 subunits to test their effect on cytokine production. 
      In both cells, after LPS treatment, overexpression of RelB and p50 inhibited the 
      production of some pro-inflammatory cytokines e.g., TNF, but not of others e.g. 
      IL6. Anti-inflammatory IL10 was not affected. Moreover, when overexpressing p50 
      alone, IL10 was increased in LPS-activated macrophages. We thus demonstrated that 
      the dimer RelB/p50 rather than the p50/p50 complex inhibits TNF production in 
      LPS-stimulated DCs and macrophages. This implies that the non-canonical RelB/p50 
      could modulate the canonical p65/p50 pathway.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Gasparini, Chiara
AU  - Gasparini C
AD  - Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy. 
      chiara.gasparini@burlo.trieste.it
FAU - Foxwell, Brian M
AU  - Foxwell BM
FAU - Feldmann, Marc
AU  - Feldmann M
LA  - eng
GR  - Arthritis Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130206
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 147337-75-5 (Transcription Factor RelB)
SB  - IM
MH  - Adenoviridae/metabolism
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Humans
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/drug effects/*metabolism
MH  - Protein Binding/drug effects
MH  - Transcription Factor RelA/metabolism
MH  - Transcription Factor RelB/*metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 2013/02/12 06:00
MHDA- 2013/08/28 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/10/17 00:00 [received]
PHST- 2012/12/28 00:00 [revised]
PHST- 2012/12/31 00:00 [accepted]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2013/08/28 06:00 [medline]
AID - S1043-4666(13)00027-6 [pii]
AID - 10.1016/j.cyto.2012.12.029 [doi]
PST - ppublish
SO  - Cytokine. 2013 Mar;61(3):736-40. doi: 10.1016/j.cyto.2012.12.029. Epub 2013 Feb 
      6.