PMID- 23395585
OWN - NLM
STAT- MEDLINE
DCOM- 20140212
LR  - 20181202
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 31
IP  - 14
DP  - 2013 Apr 3
TI  - Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal 
      disease virus in chickens.
PG  - 1799-805
LID - S0264-410X(13)00134-5 [pii]
LID - 10.1016/j.vaccine.2013.01.056 [doi]
AB  - Interleukin-18 (IL-18) is an important cytokine with multiple functions in innate 
      and acquired immunity. In this study, chicken IL-18 was evaluated for its 
      adjuvant effects on the protective immunity of a DNA vaccine carrying the VP243 
      gene of IBDV. Groups of 14-day-old SPF chickens were given twice at 2-week 
      intervals with 100 mug of the plasmid DNA vaccine pCAGVP243, pCAGVP243-IL-18 and 
      the blank vector pCAGGS, respectively, and challenged with vvIBDV (HLJ0504 
      strain) 2 weeks later. Chickens immunized with plasmid pCAGVP243-IL-18 carrying 
      both VP243 and IL-18 genes induced significantly higher levels of antibodies, 
      lymphocyte proliferation responses and of the cytokines IL-4 and IFN-gamma than those 
      injected with pCAGVP243 encoding the VP243 gene alone. Furthermore, 
      pCAGVP243-IL-18 provided higher protection (93%) against vvIBDV challenge in 
      chickens than pCAGVP243 (60%), as evidenced by the absence of clinical signs, 
      mortality, and bursal atrophy. These results indicated that the cytokine IL-18 
      could enhance the immune responses and protection efficacy of DNA vaccine against 
      IBDV infection in chickens, highlighting the potential value of chicken IL-18 as 
      an adjuvant in the prevention of vvIBDV infection.
CI  - Copyright (c) 2013. Published by Elsevier Ltd.
FAU - Li, Kai
AU  - Li K
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of 
      Agricultural Sciences, 427 Maduan Street, Harbin 150001, PR China.
FAU - Gao, Honglei
AU  - Gao H
FAU - Gao, Li
AU  - Gao L
FAU - Qi, Xiaole
AU  - Qi X
FAU - Gao, Yulong
AU  - Gao Y
FAU - Qin, Liting
AU  - Qin L
FAU - Wang, Yongqiang
AU  - Wang Y
FAU - Wang, Xiaomei
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130207
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Interleukin-18)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Animals
MH  - Antibodies, Viral/genetics/immunology
MH  - Birnaviridae Infections/immunology/prevention & control/*veterinary
MH  - *Chickens
MH  - Infectious bursal disease virus/immunology
MH  - Interleukin-18/administration & dosage/*immunology
MH  - Lymphocytes/cytology
MH  - Poultry Diseases/immunology/*prevention & control
MH  - Specific Pathogen-Free Organisms
MH  - Vaccines, DNA/genetics/immunology/therapeutic use
MH  - Viral Vaccines/genetics/immunology/therapeutic use
EDAT- 2013/02/12 06:00
MHDA- 2014/02/13 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/01/14 00:00 [revised]
PHST- 2013/01/25 00:00 [accepted]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2014/02/13 06:00 [medline]
AID - S0264-410X(13)00134-5 [pii]
AID - 10.1016/j.vaccine.2013.01.056 [doi]
PST - ppublish
SO  - Vaccine. 2013 Apr 3;31(14):1799-805. doi: 10.1016/j.vaccine.2013.01.056. Epub 
      2013 Feb 7.