PMID- 23396243
OWN - NLM
STAT- MEDLINE
DCOM- 20140314
LR  - 20130416
IS  - 1523-6536 (Electronic)
IS  - 1083-8791 (Linking)
VI  - 19
IP  - 5
DP  - 2013 May
TI  - Long-term immune reconstitution of naive and memory t cell pools after 
      haploidentical hematopoietic stem cell transplantation.
PG  - 703-12
LID - S1083-8791(13)00053-0 [pii]
LID - 10.1016/j.bbmt.2013.01.017 [doi]
AB  - Haploidentical hematopoietic stem cell transplantation (HSCT) constitutes an 
      important alternative for patients lacking a human leukocyte antigen 
      (HLA)-matched donor. Although the use of haploidentical donors is increasingly 
      common, the long-term impact of generating a donor-derived immune system in the 
      context of an HLA-mismatched thymic environment remains poorly characterized. We 
      performed an in-depth assessment of immune reconstitution in a group of 
      haploidentical HSCT recipients 4 to 6 years posttransplantation, in parallel with 
      the respective parental donors and age-matched healthy control subjects. Our data 
      show that the proportion of naive and memory subsets in the recipients, both 
      within CD8(+) and CD4(+) T cells, more closely resembled that observed in 
      age-matched control subjects than in the donors. HSCT recipients displayed 
      relatively high signal-joint T cell-receptor excision circle levels and a high 
      frequency of the recent thymic emigrant-enriched CD31(+) subset within naive 
      CD4(+) and naive regulatory T cells. Moreover, CD8(+), CD4(+), and regulatory T 
      cells from HSCT recipients displayed a diverse T cell repertoire. These results 
      support a key role for thymic output in T cell reconstitution. Nevertheless, HSCT 
      recipients had significantly shorter telomeres within a naive-enriched CD4(+) T 
      cell population than age-matched control subjects, despite the similar telomere 
      length observed within the most differentiated CD8(+) and CD4(+) T cell subsets. 
      Overall, our data suggest that long-term immune reconstitution was successfully 
      achieved after haploidentical HSCT, a process that appears to have largely relied 
      on de novo T cell production.
CI  - Copyright (c) 2013 American Society for Blood and Marrow Transplantation. Published 
      by Elsevier Inc. All rights reserved.
FAU - Azevedo, Rita I
AU  - Azevedo RI
AD  - Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 
      Lisbon, Portugal.
FAU - Soares, Maria V D
AU  - Soares MV
FAU - Albuquerque, Adriana S
AU  - Albuquerque AS
FAU - Tendeiro, Rita
AU  - Tendeiro R
FAU - Soares, Rui S
AU  - Soares RS
FAU - Martins, Miguel
AU  - Martins M
FAU - Ligeiro, Dario
AU  - Ligeiro D
FAU - Victorino, Rui M M
AU  - Victorino RM
FAU - Lacerda, Joao F
AU  - Lacerda JF
FAU - Sousa, Ana E
AU  - Sousa AE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130208
PL  - United States
TA  - Biol Blood Marrow Transplant
JT  - Biology of blood and marrow transplantation : journal of the American Society for 
      Blood and Marrow Transplantation
JID - 9600628
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Anemia, Aplastic/immunology/surgery
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cross-Sectional Studies
MH  - Female
MH  - HLA Antigens/*immunology
MH  - Haploidy
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Humans
MH  - Immunologic Memory
MH  - Leukemia/immunology/surgery
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocyte Subsets/*immunology
MH  - Tissue Donors
MH  - Transplantation Immunology
MH  - Transplantation, Homologous
MH  - Young Adult
EDAT- 2013/02/12 06:00
MHDA- 2014/03/15 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/10/12 00:00 [received]
PHST- 2013/01/19 00:00 [accepted]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2014/03/15 06:00 [medline]
AID - S1083-8791(13)00053-0 [pii]
AID - 10.1016/j.bbmt.2013.01.017 [doi]
PST - ppublish
SO  - Biol Blood Marrow Transplant. 2013 May;19(5):703-12. doi: 
      10.1016/j.bbmt.2013.01.017. Epub 2013 Feb 8.