PMID- 23397092
OWN - NLM
STAT- MEDLINE
DCOM- 20131105
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 405
IP  - 12
DP  - 2013 May
TI  - Simultaneous determination of opiates, methadone, amphetamines, cocaine, and 
      metabolites in human placenta and umbilical cord by LC-MS/MS.
PG  - 4295-305
LID - 10.1007/s00216-013-6784-6 [doi]
AB  - LC-MS/MS methods for the quantification of morphine, morphine-3-glucuronide, 
      morphine-6-glucuronide, codeine, 6-acetylmorphine, cocaine, benzoylecgonine, 
      ecgonine methyl ester, hydroxybenzoylecgonine, cocaethylene, amphetamine, 
      methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine 
      (MDMA), methadone, and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in human 
      placenta and umbilical cord were developed and validated. Specimens (1 +/- 0.02 g) 
      were homogenized with the Ultra-Turrax T8 disperser and centrifuged, and the 
      supernatant was submitted to solid-phase extraction with Oasis MCX cartridges. 
      Chromatographic separation was performed using an Atlantis T3 analytical column 
      (100 x 2.1 mm, 3 mum) and a gradient of 0.1 % formic acid and acetonitrile. 
      Selectivity was verified in 10 different blank specimens. The method was linear 
      from 1-5 to 100-500 ng/g, depending on the analyte. Limits of detection and 
      quantification ranged from 0.5 to 2.5 ng/g and 1 to 5 ng/g, respectively. Method 
      imprecision was </=15.3 %, except for MDMA at low quality control (18.1 %); 
      accuracy, 87.1 to 114 %; extraction efficiency, 16.3 to 154.0 % (%CV = 1.8-39.4 
      %); matrix effect, -75.7 to 449.9 % (%CV = 3.5-50 %); and process efficiency, 8.7 
      to 316.0 %. The method was applied to authentic placenta and umbilical cord 
      specimens from drug-user pregnant women.
FAU - de Castro, Ana
AU  - de Castro A
AD  - Toxicology Service, Institute of Forensic Sciences, University of Santiago de 
      Compostela, Santiago de Compostela, Spain. ana.decastro@usc.es
FAU - Diaz, Ariana
AU  - Diaz A
FAU - Pineiro, Beatriz
AU  - Pineiro B
FAU - Lendoiro, Elena
AU  - Lendoiro E
FAU - Cruz, Angelines
AU  - Cruz A
FAU - Lopez-Rivadulla, Manuel
AU  - Lopez-Rivadulla M
FAU - Concheiro, Marta
AU  - Concheiro M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20130210
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Analgesics, Opioid)
RN  - CK833KGX7E (Amphetamine)
RN  - I5Y540LHVR (Cocaine)
RN  - UC6VBE7V1Z (Methadone)
SB  - IM
MH  - Amphetamine/*analysis/metabolism
MH  - Analgesics, Opioid/*analysis/metabolism
MH  - Chromatography, Liquid/methods
MH  - Cocaine/*analysis/metabolism
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Methadone/*analysis/metabolism
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Solid Phase Extraction/methods
MH  - Substance Abuse Detection/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Umbilical Cord/*metabolism
EDAT- 2013/02/12 06:00
MHDA- 2013/11/06 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/10/16 00:00 [received]
PHST- 2013/01/24 00:00 [accepted]
PHST- 2012/12/19 00:00 [revised]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2013/11/06 06:00 [medline]
AID - 10.1007/s00216-013-6784-6 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2013 May;405(12):4295-305. doi: 10.1007/s00216-013-6784-6. 
      Epub 2013 Feb 10.