PMID- 23397251
OWN - NLM
STAT- MEDLINE
DCOM- 20130924
LR  - 20211021
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 175
IP  - 3-4
DP  - 2013 Apr
TI  - Patients with chronic-form paracoccidioidomycosis present high serum levels of 
      IgE anti-paracoccidioides brasiliensis Gp70.
PG  - 307-13
LID - 10.1007/s11046-013-9624-5 [doi]
AB  - Paracoccidioidomycosis (PCM) is a disease caused by the Paracoccidioides genus, 
      which includes P. brasiliensis and the new phylogenetic species P. lutzii. 
      Resistance to this infection has been correlated with a Th1 pattern of cellular 
      immune response, while susceptibility is correlated to an intense humoral immune 
      response with an increase in IgE levels. Serum levels of IgE and IgG anti-gp70 
      and anti-exoantigen in chronic PCM were analyzed by enzyme-linked immunosorbent 
      assay. Results showed a higher gp70 concentration in somatic antigen (SA) than in 
      cell-free antigen (CFA) preparation and significantly higher levels of IgE and 
      IgG anti-gp70 in chronic PCM patients' serum (n = 12) than in normal human serum 
      (n = 12) (p < 0.05). Pearson's correlation analysis showed a strong correlation 
      between IgG and IgE anti-gp70 (r = 0.8424). Additionally, IgE purified from a 
      pool of acute and chronic PCM patient's serum was analyzed by immunoblotting. The 
      patients with the acute form of the disease showed strong bands for gp43 and gp70 
      in SA but only for gp43 in CFA. In patients with the chronic form, solely the 
      gp43 band was observed. In conclusion, we found that SA is a better source of 
      gp70 than CFA is, and chronic PCM patients show high levels of IgE anti-gp70. 
      This finding suggests that the Th2 immune response is potentially induced by gp70 
      in PCM disease, which calls for further study.
FAU - Rigobello, F F
AU  - Rigobello FF
AD  - Department of Pathologic Sciences, State University of Londrina (UEL), Londrina, 
      PR, Brazil. fabianarigobello@hotmail.com
FAU - Marquez, A S
AU  - Marquez AS
FAU - Lopes, J D
AU  - Lopes JD
FAU - Nakanishi-Ito, F A
AU  - Nakanishi-Ito FA
FAU - Itano, E N
AU  - Itano EN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130209
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Fungal/*blood
MH  - Antigens, Fungal/immunology
MH  - Chronic Disease
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/*blood
MH  - Immunoglobulin G/blood
MH  - Male
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology
MH  - Serum/immunology
EDAT- 2013/02/12 06:00
MHDA- 2013/09/26 06:00
CRDT- 2013/02/12 06:00
PHST- 2012/06/28 00:00 [received]
PHST- 2013/01/25 00:00 [accepted]
PHST- 2013/02/12 06:00 [entrez]
PHST- 2013/02/12 06:00 [pubmed]
PHST- 2013/09/26 06:00 [medline]
AID - 10.1007/s11046-013-9624-5 [doi]
PST - ppublish
SO  - Mycopathologia. 2013 Apr;175(3-4):307-13. doi: 10.1007/s11046-013-9624-5. Epub 
      2013 Feb 9.