PMID- 23397388 OWN - NLM STAT- MEDLINE DCOM- 20130801 LR - 20211021 IS - 1940-6029 (Electronic) IS - 1064-3745 (Print) IS - 1064-3745 (Linking) VI - 979 DP - 2013 TI - Analyses of programmed cell death in dendritic cells. PG - 51-63 LID - 10.1007/978-1-62703-290-2_6 [doi] AB - Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses. FAU - Chen, Min AU - Chen M AD - Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA. FAU - Huang, Lily AU - Huang L FAU - Wang, Jin AU - Wang J LA - eng GR - R01 AI074949-05/AI/NIAID NIH HHS/United States GR - R01 DK083164-05/DK/NIDDK NIH HHS/United States GR - R01 GM087710/GM/NIGMS NIH HHS/United States GR - R01 AI056210/AI/NIAID NIH HHS/United States GR - R01 DK083164-04/DK/NIDDK NIH HHS/United States GR - R01AI074949/AI/NIAID NIH HHS/United States GR - R01 GM087710-09/GM/NIGMS NIH HHS/United States GR - R01DK083164/DK/NIDDK NIH HHS/United States GR - R01AI056210/AI/NIAID NIH HHS/United States GR - R01 AI074949/AI/NIAID NIH HHS/United States GR - R01 DK083164/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (Peptides) RN - G34N38R2N1 (Bromodeoxyuridine) SB - IM MH - Adoptive Transfer MH - Animals MH - *Apoptosis MH - Bone Marrow Cells/cytology MH - Bromodeoxyuridine/metabolism MH - CD4-Positive T-Lymphocytes/cytology/immunology MH - CD8-Positive T-Lymphocytes/cytology/immunology MH - Cell Separation MH - Cytological Techniques/*methods MH - Dendritic Cells/*cytology/immunology MH - Lymphocyte Activation MH - Mice MH - Peptides/immunology MH - Spleen/cytology PMC - PMC3576849 MID - NIHMS358585 EDAT- 2013/02/12 06:00 MHDA- 2013/08/02 06:00 PMCR- 2014/01/01 CRDT- 2013/02/12 06:00 PHST- 2013/02/12 06:00 [entrez] PHST- 2013/02/12 06:00 [pubmed] PHST- 2013/08/02 06:00 [medline] PHST- 2014/01/01 00:00 [pmc-release] AID - 10.1007/978-1-62703-290-2_6 [doi] PST - ppublish SO - Methods Mol Biol. 2013;979:51-63. doi: 10.1007/978-1-62703-290-2_6.