PMID- 23398265
OWN - NLM
STAT- MEDLINE
DCOM- 20130918
LR  - 20161018
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Linking)
VI  - 27
IP  - 2
DP  - 2013 Mar-Apr
TI  - Assessment of serum myokines and markers of inflammation associated with exercise 
      in endurance racing sled dogs.
PG  - 371-6
LID - 10.1111/jvim.12046 [doi]
AB  - BACKGROUND: The endurance sled dog is the ultimate endurance athlete in which to 
      examine the exercise-associated acute phase and myokine responses that might be 
      related to changes in muscle metabolism and damage. An inciting cause for 
      increased C-reactive protein has yet to be elucidated, which might involve 
      interleukin-6 and other myokines. OBJECTIVES: To examine concentrations of 
      interleukin-6 (IL-6), interleukin-15 (IL-15), interleukin-8 (IL-8), and tumor 
      necrosis factor-alpha (TNF-alpha), and markers of the inflammatory response of exercise; 
      monocyte chemoattractant protein-1(MCP-1) and C-reactive protein (CRP) before, 
      during, and after an endurance racing event. ANIMALS: 26 sled dogs completing a 
      1650-km race. METHODS: In a prospective study, cephalic venipuncture was 
      performed before racing, at the midpoint, and after racing. Body weight and serum 
      CRP, MCP-1, IL-15, IL-8, IL-6, and TNF-alpha concentrations were evaluated using 
      enzyme-linked immunoabsorbance-based assays or a luminex multiplex assay. 
      RESULTS: There were no significant differences in concentrations of IL-6, IL-15, 
      IL-8, or TNF-alpha at the 3 time points, whereas there were significant increases in 
      MCP-1 (median and range-start: 86 pg/mL [30-1845]; midpoint: 179 pg/mL [53-730]; 
      finish: 180 pg/mL [21-1294]; P < .01) and CRP (median and range-start: 18 mug/mL 
      [11-58]; midpoint: 76 mug/mL [12-198]; finish: 60 mug/mL [12-170]; P < .01) at the 
      midpoint and race finish. There was a significant linear relationship between 
      MCP-1 and IL-6 (R = 0.68; P < .01). CONCLUSIONS AND CLINICAL SIGNIFICANCE: The 
      inflammatory response to exercise increases as measured by MCP-1 during and after 
      endurance exercise in sled dogs. IL-6 appears to be associated with MCP-1; 
      however, the reasons for increases in the acute phase response (CRP) cannot be 
      attributed to IL-6 or other myokines. IL-6 and MCP-1 concentrations might be 
      useful in future investigations of exertional rhabdomyolysis.
CI  - Copyright (c) 2013 by the American College of Veterinary Internal Medicine.
FAU - Yazwinski, M
AU  - Yazwinski M
AD  - Department of Clinical Sciences, Cornell University College of Veterinary 
      Medicine, Ithaca, NY 14853, USA.
FAU - Milizio, J G
AU  - Milizio JG
FAU - Wakshlag, J J
AU  - Wakshlag JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130211
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - C-Reactive Protein/metabolism
MH  - Chemokine CCL2/blood
MH  - Dog Diseases/*blood/etiology
MH  - Dogs/*blood
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Inflammation/blood/*veterinary
MH  - Interleukins/blood
MH  - *Physical Endurance
MH  - Prospective Studies
MH  - Regression Analysis
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2013/02/13 06:00
MHDA- 2013/09/21 06:00
CRDT- 2013/02/13 06:00
PHST- 2012/09/19 00:00 [received]
PHST- 2012/12/10 00:00 [revised]
PHST- 2013/01/03 00:00 [accepted]
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
AID - 10.1111/jvim.12046 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2013 Mar-Apr;27(2):371-6. doi: 10.1111/jvim.12046. Epub 2013 
      Feb 11.