PMID- 23398290
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20211203
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 125
IP  - 4
DP  - 2013 May
TI  - Retinoids and glucocorticoids target common genes in hippocampal HT22 cells.
PG  - 518-31
LID - 10.1111/jnc.12192 [doi]
AB  - Vitamin A metabolite retinoic acid (RA) plays a major role in the aging adult 
      brain plasticity. Conversely, chronic excess of glucocorticoids (GC) elicits some 
      deleterious effects in the hippocampus. We questioned here the involvement of RA 
      and GC in the expression of target proteins in hippocampal neurons. We 
      investigated proteins involved either in the signaling pathways [RA receptor beta 
      (RARbeta) and glucocorticoid receptor (GR)] or in neuron differentiation and 
      plasticity [tissue transglutaminase 2 (tTG) and brain-derived neurotrophic factor 
      (BDNF)] in a hippocampal cell line, HT22. We applied RA and/or dexamethasone 
      (Dex) as activators of the pathways and investigated mRNA and protein expression 
      of their receptors and of tTG and BDNF as well as tTG activity and BDNF 
      secretion. Our results confirm the involvement of RA- and GC-dependent pathways 
      and their interaction in our neuronal cell model. First, both pathways regulate 
      the transcription and expression of own and reciprocal receptors: RA and Dex 
      increased RARbeta and decreased GR expressions. Second, Dex reduces the expression 
      of tTG when associated with RA despite stimulating its expression when used 
      alone. Importantly, when they are combined, RA counteracts the deleterious effect 
      of glucocorticoids on BDNF regulation and thus may improve neuronal plasticity 
      under stress conditions. In conclusion, GC and RA both interact through 
      regulations of the two receptors, RARbeta and GR. Furthermore, they both act, 
      synergistically or oppositely, on other target proteins critical for neuronal 
      plasticity, tTG and BDNF.
CI  - (c) 2013 International Society for Neurochemistry.
FAU - Brossaud, Julie
AU  - Brossaud J
AD  - INRA, Nutrition et Neurobiologie Integree, UMR1286, Bordeaux, France.
FAU - Roumes, Helene
AU  - Roumes H
FAU - Moisan, Marie-Pierre
AU  - Moisan MP
FAU - Pallet, Veronique
AU  - Pallet V
FAU - Redonnet, Anabelle
AU  - Redonnet A
FAU - Corcuff, Jean-Benoit
AU  - Corcuff JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130306
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glucocorticoids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (retinoic acid receptor beta)
RN  - 5688UTC01R (Tretinoin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Aging/physiology
MH  - Animals
MH  - Apoptosis/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Line, Transformed
MH  - Cell Survival/drug effects/physiology
MH  - Dexamethasone/metabolism/*pharmacology
MH  - Drug Synergism
MH  - GTP-Binding Proteins/genetics/metabolism
MH  - Gene Expression/drug effects/physiology
MH  - Glucocorticoids/metabolism/*pharmacology
MH  - Hippocampus/*cytology
MH  - Mice
MH  - Necrosis
MH  - Neural Stem Cells/cytology/*drug effects
MH  - Neuronal Plasticity/drug effects/physiology
MH  - Protein Glutamine gamma Glutamyltransferase 2
MH  - RNA, Messenger/metabolism
MH  - Receptors, Glucocorticoid/genetics/metabolism
MH  - Receptors, Retinoic Acid/genetics/metabolism
MH  - Signal Transduction/*drug effects/physiology
MH  - Transglutaminases/genetics/metabolism
MH  - Tretinoin/metabolism/*pharmacology
EDAT- 2013/02/13 06:00
MHDA- 2013/07/03 06:00
CRDT- 2013/02/13 06:00
PHST- 2012/11/15 00:00 [received]
PHST- 2013/01/11 00:00 [revised]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.1111/jnc.12192 [doi]
PST - ppublish
SO  - J Neurochem. 2013 May;125(4):518-31. doi: 10.1111/jnc.12192. Epub 2013 Mar 6.