PMID- 23398398
OWN - NLM
STAT- MEDLINE
DCOM- 20140319
LR  - 20161125
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 47
IP  - 6
DP  - 2013 Mar 19
TI  - Biliary PAH and alkylphenol metabolites, biomarker enzyme activities, and gene 
      expression levels in the deep-sea fish Alepocephalus rostratus.
PG  - 2854-61
LID - 10.1021/es304345s [doi]
AB  - Biliary polycyclic aromatic hydrocarbon (PAH) and alkylphenol (AP) metabolites, 
      hepatic gene expression, and corresponding enzyme activities were determined in 
      the deep-sea fish Alepocephalus rostratus from two sites within the 
      Mediterranean. Biliary metabolites included the hydroxylated PAH metabolites 
      (OH-PAHs) 1-naphthol, 2-naphthol, 9-fluorenol, 9-phenanthrol, and 1-pyrenol and 
      the APs 4-nonylphenol (NP) and 4-tert-octylphenol (OP). Five biomarker genes, 
      namely, cytochrome P450 1A (CYP1A), vitellogenin (Vtg), catalase (CAT), 
      Cu/Zn-superoxide-dismutase (Cu/Zn-SOD), and glutathione reductase (GR), were 
      quantified using qRT-PCR. Moreover, corresponding enzyme activities 
      (ethoxyresorufin-O-deethylase (EROD), CAT, SOD, and GR) were also determined. The 
      SigmaOH-PAHs detected ranged from 21.1 to 300.3 ng/g bile and were mainly composed of 
      1-naphthol. Both NP and OP metabolites were detected in all samples with 
      concentrations ranging from 17.4 to 107.2 ng/g bile and 4.9 to 17.3 ng/g bile, 
      respectively, and levels were significantly higher in samples from the western 
      Mediterranean (WM) compared to those from the Catalan slope (CS). Accordingly, 
      gene expression was significantly induced in male fish from the WM; however, 
      these results were not reflected in enzyme activity levels. In particular, males 
      caught at 2000 m in the WM exhibited 35-times higher Vtg levels compared to those 
      from the CS, suggesting that endocrine-disrupting effects may potentially be 
      occurring in such remote environments as the deep-sea.
FAU - Koenig, Samuel
AU  - Koenig S
AD  - Institute of Marine Science (ICM-CSIC), Passeig Maritim de la Barceloneta 37-49, 
      08003 Barcelona, Catalonia, Spain. koenig@icm.csic.es
FAU - Porte, Cinta
AU  - Porte C
FAU - Sole, Montserrat
AU  - Sole M
FAU - Sturve, Joachim
AU  - Sturve J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130225
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Phenols)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Bile/*metabolism
MH  - Female
MH  - Fishes/genetics/*metabolism
MH  - Gene Expression
MH  - Liver/*enzymology/metabolism
MH  - Male
MH  - Phenols/*metabolism
MH  - Polycyclic Aromatic Hydrocarbons/*metabolism
MH  - Water Pollutants, Chemical/*metabolism
EDAT- 2013/02/13 06:00
MHDA- 2014/03/22 06:00
CRDT- 2013/02/13 06:00
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
AID - 10.1021/es304345s [doi]
PST - ppublish
SO  - Environ Sci Technol. 2013 Mar 19;47(6):2854-61. doi: 10.1021/es304345s. Epub 2013 
      Feb 25.