PMID- 23398508
OWN - NLM
STAT- MEDLINE
DCOM- 20130917
LR  - 20211021
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 81
IP  - 3
DP  - 2013 Mar
TI  - A one-step DNA sequencing strategy to HLA type hematopoietic stem cell donors at 
      recruitment - rethinking typing strategies.
PG  - 150-60
LID - 10.1111/tan.12072 [doi]
AB  - In order to reduce the time required to identify a match for unrelated donor 
      hematopoietic stem cell transplantation, a one-step DNA sequencing strategy was 
      employed at the time of recruitment. The impact of this strategy on human 
      leukocyte antigen (HLA) typing resolution and the effect of current registry 
      requirements on resolution and coding of assignments were evaluated. Sanger-based 
      DNA sequencing was used to obtain diploid exons 2 and 3 HLA-A, -B and -C 
      assignments of 2747 unrelated African American and 1822 European American 
      volunteers at recruitment. The results demonstrate the high resolution of the 
      approach and challenge several aspects of the current registry typing strategy. 
      Of the 46% of African American and 74% of European American individuals whose HLA 
      typing resulted in alternative genotypes, the majority (>/=93%) was predicted to 
      have only a single 'common' genotype among the alternatives. The common practice 
      of adding secondary assays to resolve alternative genotype assignments that 
      include more than two antigen groups was also evaluated. While the percentage of 
      assignments with greater than two antigen groups reached as high as 21% (HLA-A in 
      European Americans), only 1.8% of individuals at most carried two common 
      genotypes encompassing three antigen groups. The assignment of (National Marrow 
      Donor Program) NMDP-designated allele codes to the one-pass results reduced the 
      resolution substantially and introduced genotypes that were not included in the 
      laboratory's assignments. We suggest the alternative strategy of using the exons 
      2-3 diploid nucleotide sequence as the assignment submitted to the registry with 
      the added benefit of immortalizing the assignment in time regardless of the 
      introduction of novel alleles. To keep pace with current donor selection criteria 
      and with the increasing number of new alleles, it is time to rethink our 
      recruitment typing strategies.
CI  - (c) 2013 John Wiley & Sons A/S.
FAU - Tu, B
AU  - Tu B
AD  - Department of Pediatrics, CW Bill Young Marrow Donor Recruitment and Research 
      Program, Georgetown University, Washington, DC, USA.
FAU - Cha, N
AU  - Cha N
FAU - Yang, R
AU  - Yang R
FAU - Ng, J
AU  - Ng J
FAU - Hurley, C K
AU  - Hurley CK
LA  - eng
GR  - P30 CA051008/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - Diploidy
MH  - Gene Frequency/genetics
MH  - Genotype
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - *Patient Selection
MH  - Sequence Analysis, DNA/*methods
MH  - *Tissue Donors
PMC - PMC4189109
MID - NIHMS629022
COIS- Conflict of Interest The authors have no conflicts of interest.
EDAT- 2013/02/13 06:00
MHDA- 2013/09/18 06:00
PMCR- 2014/10/08
CRDT- 2013/02/13 06:00
PHST- 2012/10/19 00:00 [received]
PHST- 2012/12/27 00:00 [revised]
PHST- 2013/01/21 00:00 [accepted]
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/09/18 06:00 [medline]
PHST- 2014/10/08 00:00 [pmc-release]
AID - 10.1111/tan.12072 [doi]
PST - ppublish
SO  - Tissue Antigens. 2013 Mar;81(3):150-60. doi: 10.1111/tan.12072.