PMID- 23399301
OWN - NLM
STAT- MEDLINE
DCOM- 20131030
LR  - 20141120
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 91
IP  - 8
DP  - 2013 May
TI  - Use of effect-directed analysis for the identification of organic toxicants in 
      surface flow constructed wetland sediments.
PG  - 1165-75
LID - S0045-6535(13)00100-8 [pii]
LID - 10.1016/j.chemosphere.2013.01.023 [doi]
AB  - Wetlands constitute one of the most efficient ecosystems with a great capacity to 
      recycle the organic matter and able to attenuate or mitigate the chemical 
      pollution. However, limited information exists on the ecotoxicological effects 
      that may be caused due to the presence of these pollutants in wetland sediments. 
      In this work, a bioassay-directed approach was used to identify toxicologically 
      active compounds retained in sediments from a surface flow constructed wetland 
      located in the North-Eastern of Spain. Sediment fractionation was accomplished by 
      pressurized-liquid extraction (PLE) followed by semipreparative normal phase high 
      performance liquid chromatography (NP-HPLC). During the extraction procedure, 
      different solvents were sequentially applied in order to selectively extract the 
      compounds as a function of their polarity. The cytotoxicity of the resulting 
      fractions was assessed on the fish hepatoma cell line PLHC-1 by using the 
      thiazolyl blue tetrazolium bromide (MTT) assay, while the presence of CYP1A 
      inducing agents was determined by measuring the activity 
      7-ethoxyresorufin-O-deethylase (EROD) in exposed cells. Identification of the 
      compounds was performed by gas chromatography coupled to mass spectrometry 
      (GC/MS). Compounds such as polycyclic aromatic hydrocarbons (PAHs), non-steroidal 
      anti-inflammatory drugs (NSAIDs), polycyclic musk fragrances and pesticides were 
      identified in the most toxic fractions.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Regueiro, Jorge
AU  - Regueiro J
AD  - Nutrition and Bromatology Group, Analytical and Food Chemistry Department, 
      Faculty of Food Science and Technology, Ourense Campus, University of Vigo, 
      E-32004 Ourense, Spain. jorge.regueiro@uvigo.es
FAU - Matamoros, Victor
AU  - Matamoros V
FAU - Thibaut, Remi
AU  - Thibaut R
FAU - Porte, Cinta
AU  - Porte C
FAU - Bayona, Josep M
AU  - Bayona JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130208
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Organic Chemicals)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - *Environmental Monitoring
MH  - Environmental Restoration and Remediation
MH  - Geologic Sediments/*chemistry
MH  - Organic Chemicals/*analysis/toxicity
MH  - Spain
MH  - Waste Disposal, Fluid
MH  - Water Pollutants, Chemical/*analysis/toxicity
MH  - *Wetlands
EDAT- 2013/02/13 06:00
MHDA- 2013/10/31 06:00
CRDT- 2013/02/13 06:00
PHST- 2012/05/25 00:00 [received]
PHST- 2012/11/28 00:00 [revised]
PHST- 2013/01/02 00:00 [accepted]
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/10/31 06:00 [medline]
AID - S0045-6535(13)00100-8 [pii]
AID - 10.1016/j.chemosphere.2013.01.023 [doi]
PST - ppublish
SO  - Chemosphere. 2013 May;91(8):1165-75. doi: 10.1016/j.chemosphere.2013.01.023. Epub 
      2013 Feb 8.