PMID- 23399837
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR  - 20191210
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 280
IP  - 2
DP  - 2012 Dec
TI  - Amino acid at position 176 was essential for porcine reproductive and respiratory 
      syndrome virus (PRRSV) non-structural protein 1alpha (nsp1alpha) as an inhibitor to the 
      induction of IFN-beta.
PG  - 125-31
LID - S0008-8749(12)00187-6 [pii]
LID - 10.1016/j.cellimm.2012.10.003 [doi]
AB  - Previous studies have shown that porcine reproductive and respiratory syndrome 
      virus (PRRSV) nonstructural protein 1alpha (nsp1alpha) was the interferon (IFN) 
      antagonist. However, the mechanism was unclear. In the present study, deletion of 
      the carboxyl-terminal extension (CTE) (167-180 amino acid (aa)) made nsp1alpha lose 
      its inhibitory ability to the induction of IFN-beta. And a series of C-terminal 
      truncated mutants for nsp1alpha showed that 1-176 aa of nsp1alpha was able to inhibit the 
      induction of IFN-beta and deleting or mutating the amino acid F176 made nsp1alpha not 
      inhibit the induction of IFN-beta. In conclusion, the CTE and the amino acid F176 
      were critical for nsp1alpha as the IFN antagonist and the region representing 167-176 
      was the minimal subunit of the CTE for nsp1alpha to retain its suppressive activity 
      to the induction of IFN-beta.
CI  - Copyright (c) 2012. Published by Elsevier Inc.
FAU - Shi, Xibao
AU  - Shi X
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China.
FAU - Chen, Jing
AU  - Chen J
FAU - Xing, Guangxu
AU  - Xing G
FAU - Zhang, Xiaozhuan
AU  - Zhang X
FAU - Hu, Xiaofei
AU  - Hu X
FAU - Zhi, Yubao
AU  - Zhi Y
FAU - Guo, Junqing
AU  - Guo J
FAU - Wang, Li
AU  - Wang L
FAU - Qiao, Songlin
AU  - Qiao S
FAU - Lu, Qingxia
AU  - Lu Q
FAU - Zhang, Gaiping
AU  - Zhang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121029
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Viral Nonstructural Proteins)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Immunity, Innate
MH  - Interferon-beta/*antagonists & inhibitors/biosynthesis
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*chemistry
MH  - Structure-Activity Relationship
MH  - Swine
MH  - Viral Nonstructural Proteins/chemistry/*physiology
EDAT- 2013/02/13 06:00
MHDA- 2013/05/08 06:00
CRDT- 2013/02/13 06:00
PHST- 2012/08/10 00:00 [received]
PHST- 2012/10/18 00:00 [accepted]
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
AID - S0008-8749(12)00187-6 [pii]
AID - 10.1016/j.cellimm.2012.10.003 [doi]
PST - ppublish
SO  - Cell Immunol. 2012 Dec;280(2):125-31. doi: 10.1016/j.cellimm.2012.10.003. Epub 
      2012 Oct 29.