PMID- 23400934
OWN - NLM
STAT- MEDLINE
DCOM- 20140303
LR  - 20211203
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 66
IP  - 3
DP  - 2013 Jul
TI  - Increased expression of the spindle checkpoint protein BubR1 is associated with 
      high cell proliferation in primary gastrointestinal diffuse large B cell 
      lymphoma.
PG  - 747-52
LID - 10.1007/s12013-013-9519-6 [doi]
AB  - Primary gastrointestinal diffuse large B cell lymphoma (PGI-DLBCL) is a 
      relatively rare malignancy with limited results on clinical characteristics and 
      carcinogenesis. Chromosome instability (CIN) is a hallmark of cancer cells, 
      including lymphoma. As a key component of spindle assembly checkpoint (SAC), 
      BubR1 plays a crucial role in maintaining genome stability. To elucidate the 
      roles of BubR1 in the pathogenesis of PGI-DLBCL, and its relationship to cell 
      proliferation, Helicobacter pylori infection, and BCL-6 gene translocation, we 
      examined the possible alterations of BubR1 protein expression in PGI-DLBCL 
      patients. Paraffin-embedded cancer tissues from 20 PGI-DLBCL patients were 
      evaluated for BubR1 and H. pylori expression by immunohistochemistry, as well as 
      cell proliferative activity measured by Ki-67 proliferation index (PI). BCL-6 
      gene rearrangement was assessed by fluorescence in situ hybridization (FISH) and 
      BubR1 expression status was compared with clinicopathological parameters in 
      PGI-DLBCL patients. Overexpression of BubR1 was observed in the majority of 
      PGI-DLBCL patients. The mean expression level of BubR1 was 57.33 +/- 23.27% in 
      PGI-DLBCL, which was higher than normal gastrointestinal tissue (10.18 +/- 5.65%) 
      and reactive lymph node (26.74 +/- 8.60%) (P < 0.01), while it was comparable to 
      nodal lymphoma (54.32 +/- 21.28%) (P > 0.05). BubR1 overexpression had a positive 
      correlation with Ki-67 proliferation index (PI) (r = 0.51, P < 0.01) in 
      PGI-DLBCL, but had no relationship to H. pylori infection and BCL-6 gene 
      translocation. In addition, no correlation was found between BubR1 expression 
      levels and overall survivals (P > 0.05). BubR1 overexpression was associated with 
      cell proliferation and may play a role in the carcinogenesis of PGI-DLBCL. 
      Aberrant BubR1 expression may potentially be a biomarker for estimating biologic 
      characteristics of PGI-DLBCL.
FAU - Chen, Fei
AU  - Chen F
AD  - Department of Gastroenterology, Zhongnan Hospital of Wuhan University School of 
      Medicine, Wuhan, People's Republic of China.
FAU - Yang, Guifang
AU  - Yang G
FAU - Xia, Bing
AU  - Xia B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (BCL6 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
RN  - EC 2.7.11.1 (BUB1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Proliferation
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Gastrointestinal Neoplasms/genetics/*metabolism/*pathology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Rearrangement
MH  - Humans
MH  - Ki-67 Antigen/metabolism
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/*metabolism/*pathology
MH  - *M Phase Cell Cycle Checkpoints
MH  - Male
MH  - Middle Aged
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-bcl-6
EDAT- 2013/02/13 06:00
MHDA- 2014/03/04 06:00
CRDT- 2013/02/13 06:00
PHST- 2013/02/13 06:00 [entrez]
PHST- 2013/02/13 06:00 [pubmed]
PHST- 2014/03/04 06:00 [medline]
AID - 10.1007/s12013-013-9519-6 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2013 Jul;66(3):747-52. doi: 10.1007/s12013-013-9519-6.