PMID- 2340104
OWN - NLM
STAT- MEDLINE
DCOM- 19900627
LR  - 20190918
IS  - 0177-3593 (Print)
IS  - 0177-3593 (Linking)
VI  - 371
IP  - 3
DP  - 1990 Mar
TI  - Gulonolactone oxidase is missing in teleost fish. The direct spectrophotometric 
      assay.
PG  - 207-14
AB  - Data in the literature imply that some fish species evolved with the capacity to 
      synthesize ascorbic acid. Gulonolactone oxidase activity has been reported in 
      kidney and/or liver tissues. However, it is shown here that this microsomal 
      enzyme activity is missing in common carp hepatopancreas and kidney, whereas high 
      activity was confirmed in pigeon kidney, rat liver, bovine liver and amphibian 
      (Xenopus) kidney tissues. A new assay using either the whole tissue homogenate or 
      microsomes solubilized by sodium deoxycholate was developed to directly measure 
      the formation of ascorbic acid spectrophotometrically. Identical values were 
      found using this assay as well as the assay in which formed ascorbate was 
      determined by the dinitrophenyl hydrazine (DNPH) method. In some experiments, 
      these results were confirmed by polarographically measured oxygen consumption.
FAU - Dabrowski, K
AU  - Dabrowski K
AD  - Zoologisches Institut, Universitat Innsbruck.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Biol Chem Hoppe Seyler
JT  - Biological chemistry Hoppe-Seyler
JID - 8503054
RN  - EC 1.1.- (Sugar Alcohol Dehydrogenases)
RN  - EC 1.1.3.8 (L-Gulonolactone Oxidase)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Ascorbic Acid/*biosynthesis
MH  - Carps/*metabolism
MH  - Cattle
MH  - Columbidae
MH  - Cyprinidae/*metabolism
MH  - Kidney/enzymology
MH  - L-Gulonolactone Oxidase
MH  - Liver/enzymology
MH  - Pancreas/enzymology
MH  - Rats
MH  - Species Specificity
MH  - Spectrophotometry
MH  - Sugar Alcohol Dehydrogenases/*analysis
MH  - Xenopus laevis
EDAT- 1990/03/01 00:00
MHDA- 1990/03/01 00:01
CRDT- 1990/03/01 00:00
PHST- 1990/03/01 00:00 [pubmed]
PHST- 1990/03/01 00:01 [medline]
PHST- 1990/03/01 00:00 [entrez]
AID - 10.1515/bchm3.1990.371.1.207 [doi]
PST - ppublish
SO  - Biol Chem Hoppe Seyler. 1990 Mar;371(3):207-14. doi: 
      10.1515/bchm3.1990.371.1.207.